Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 524947..525583 Replicon chromosome
Accession NZ_CP035395
Organism Bacillus subtilis strain SRCM103697

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag ES968_RS02635 Protein ID WP_003156187.1
Coordinates 525233..525583 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag ES968_RS02630 Protein ID WP_003225183.1
Coordinates 524947..525228 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ES968_RS02610 521306..521905 - 600 WP_041850724.1 rhomboid family intramembrane serine protease -
ES968_RS02615 522000..522365 + 366 WP_014478897.1 holo-ACP synthase -
ES968_RS02620 522531..523547 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
ES968_RS02625 523662..524831 + 1170 WP_129134189.1 alanine racemase -
ES968_RS02630 524947..525228 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
ES968_RS02635 525233..525583 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
ES968_RS02640 525698..526522 + 825 WP_015252765.1 RsbT co-antagonist protein RsbRA -
ES968_RS02645 526527..526892 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
ES968_RS02650 526896..527297 + 402 WP_129134190.1 serine/threonine-protein kinase RsbT -
ES968_RS02655 527309..528316 + 1008 WP_014478900.1 phosphoserine phosphatase RsbU -
ES968_RS02660 528385..528714 + 330 WP_014475877.1 anti-sigma factor antagonist RsbV -
ES968_RS02665 528711..529193 + 483 WP_095010756.1 anti-sigma B factor RsbW -
ES968_RS02670 529159..529947 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
ES968_RS02675 529947..530546 + 600 WP_129134191.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T117741 WP_003156187.1 NZ_CP035395:525233-525583 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T117741 NZ_CP035395:525233-525583 [Bacillus subtilis]
TTGATTGTGAAACGAGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT117741 WP_003225183.1 NZ_CP035395:524947-525228 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT117741 NZ_CP035395:524947-525228 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References