Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 512626..513262 Replicon chromosome
Accession NZ_CP035394
Organism Bacillus subtilis strain SRCM103696

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag ES967_RS02620 Protein ID WP_003156187.1
Coordinates 512912..513262 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag ES967_RS02615 Protein ID WP_003225183.1
Coordinates 512626..512907 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ES967_RS02595 508985..509584 - 600 WP_032722928.1 rhomboid family intramembrane serine protease -
ES967_RS02600 509679..510044 + 366 WP_015252768.1 holo-ACP synthase -
ES967_RS02605 510210..511226 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
ES967_RS02610 511341..512510 + 1170 WP_015252766.1 alanine racemase -
ES967_RS02615 512626..512907 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
ES967_RS02620 512912..513262 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
ES967_RS02625 513377..514201 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
ES967_RS02630 514206..514571 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
ES967_RS02635 514575..514976 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
ES967_RS02640 514988..515995 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
ES967_RS02645 516057..516386 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
ES967_RS02650 516383..516865 + 483 WP_003234299.1 anti-sigma B factor RsbW -
ES967_RS02655 516831..517619 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
ES967_RS02660 517619..518218 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T117736 WP_003156187.1 NZ_CP035394:512912-513262 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T117736 NZ_CP035394:512912-513262 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT117736 WP_003225183.1 NZ_CP035394:512626-512907 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT117736 NZ_CP035394:512626-512907 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References