Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 493890..494527 Replicon chromosome
Accession NZ_CP035393
Organism Bacillus velezensis strain SRCM103691

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag ES966_RS02510 Protein ID WP_003156187.1
Coordinates 494177..494527 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag ES966_RS02505 Protein ID WP_003156188.1
Coordinates 493890..494171 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ES966_RS02485 490256..490854 - 599 Protein_441 rhomboid family intramembrane serine protease -
ES966_RS02490 490947..491312 + 366 WP_007609580.1 holo-ACP synthase -
ES966_RS02495 491477..492484 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
ES966_RS02500 492601..493770 + 1170 WP_025649966.1 alanine racemase -
ES966_RS02505 493890..494171 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
ES966_RS02510 494177..494527 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
ES966_RS02515 494645..495466 + 822 WP_025649964.1 STAS domain-containing protein -
ES966_RS02520 495471..495836 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
ES966_RS02525 495839..496240 + 402 WP_003156178.1 anti-sigma regulatory factor -
ES966_RS02530 496252..497258 + 1007 Protein_450 PP2C family protein-serine/threonine phosphatase -
ES966_RS02535 497322..497663 + 342 WP_206734249.1 anti-sigma factor antagonist -
ES966_RS02540 497647..498129 + 483 WP_007609591.1 anti-sigma B factor RsbW -
ES966_RS02545 498095..498883 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
ES966_RS02550 498883..499485 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T117734 WP_003156187.1 NZ_CP035393:494177-494527 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T117734 NZ_CP035393:494177-494527 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT117734 WP_003156188.1 NZ_CP035393:493890-494171 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT117734 NZ_CP035393:493890-494171 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References