Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 509790..510426 Replicon chromosome
Accession NZ_CP035391
Organism Bacillus subtilis strain SRCM103689

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag ES965_RS02625 Protein ID WP_003156187.1
Coordinates 510076..510426 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag ES965_RS02620 Protein ID WP_003225183.1
Coordinates 509790..510071 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ES965_RS02600 506149..506748 - 600 WP_124060063.1 rhomboid family intramembrane serine protease -
ES965_RS02605 506843..507208 + 366 WP_014475874.1 holo-ACP synthase -
ES965_RS02610 507374..508390 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
ES965_RS02615 508505..509674 + 1170 WP_015252766.1 alanine racemase -
ES965_RS02620 509790..510071 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
ES965_RS02625 510076..510426 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
ES965_RS02630 510541..511365 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
ES965_RS02635 511370..511735 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
ES965_RS02640 511739..512140 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
ES965_RS02645 512152..513159 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
ES965_RS02650 513221..513550 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
ES965_RS02655 513547..514029 + 483 WP_003234299.1 anti-sigma B factor RsbW -
ES965_RS02660 513995..514783 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
ES965_RS02665 514783..515382 + 600 WP_003234303.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T117732 WP_003156187.1 NZ_CP035391:510076-510426 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T117732 NZ_CP035391:510076-510426 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT117732 WP_003225183.1 NZ_CP035391:509790-510071 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT117732 NZ_CP035391:509790-510071 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References