Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 519499..520135 Replicon chromosome
Accession NZ_CP035390
Organism Bacillus subtilis strain SRCM103641

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag ES963_RS02680 Protein ID WP_003156187.1
Coordinates 519785..520135 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag ES963_RS02675 Protein ID WP_003225183.1
Coordinates 519499..519780 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ES963_RS02655 515858..516457 - 600 WP_129092937.1 rhomboid family intramembrane serine protease -
ES963_RS02660 516552..516917 + 366 WP_015252768.1 holo-ACP synthase -
ES963_RS02665 517083..518099 + 1017 WP_080019556.1 outer membrane lipoprotein carrier protein LolA -
ES963_RS02670 518214..519383 + 1170 WP_015252766.1 alanine racemase -
ES963_RS02675 519499..519780 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
ES963_RS02680 519785..520135 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
ES963_RS02685 520250..521074 + 825 WP_015252765.1 RsbT co-antagonist protein RsbRA -
ES963_RS02690 521079..521444 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
ES963_RS02695 521448..521849 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
ES963_RS02700 521861..522868 + 1008 WP_015252763.1 phosphoserine phosphatase RsbU -
ES963_RS02705 522930..523259 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
ES963_RS02710 523256..523738 + 483 WP_003234299.1 anti-sigma B factor RsbW -
ES963_RS02715 523704..524492 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
ES963_RS02720 524492..525091 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T117730 WP_003156187.1 NZ_CP035390:519785-520135 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T117730 NZ_CP035390:519785-520135 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT117730 WP_003225183.1 NZ_CP035390:519499-519780 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT117730 NZ_CP035390:519499-519780 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References