Detailed information of TA system TA117714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 284354..285024
• Replicon: plasmid pAP855
• Accession: NZ_CP035384
• Organism: Klebsiella pneumoniae strain AP8555

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: ES964_RS28485
• Protein ID: WP_004213072.1
• Coordinates: 284354..284797 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: ES964_RS28490
• Protein ID: WP_004213073.1
• Coordinates: 284794..285024 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ES964_RS28435	279764..280039	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
ES964_RS28440	280102..280593	+	492	WP_011251327.1	DM13 domain-containing protein	-
ES964_RS28445	280642..281562	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
ES964_RS28455	281653..282059	+	407	Protein_309	GAF domain-containing protein	-
ES964_RS29950	282574..283210	-	637	Protein_310	mucoid phenotype A regulator RmpA	-
ES964_RS28475	283627..283920	+	294	Protein_311	transposase	-
ES964_RS28480	283924..284340	+	417	Protein_312	restriction endonuclease	-
ES964_RS28485	284354..284797	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
ES964_RS28490	284794..285024	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ES964_RS28495	285632..286765	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
ES964_RS28500	286781..287074	+	294	WP_004213076.1	hypothetical protein	-
ES964_RS28505	287064..287270	-	207	WP_004213077.1	hypothetical protein	-
ES964_RS28510	287622..287912	+	291	WP_004213078.1	hypothetical protein	-
ES964_RS28515	287902..288801	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..357837	357837

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T117714 WP_004213072.1 NZ_CP035384:c284797-284354 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T117714 NZ_CP035384:c284797-284354 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT117714 WP_004213073.1 NZ_CP035384:c285024-284794 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT117714 NZ_CP035384:c285024-284794 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620