Detailed information of TA system TA117685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 4278146..4278801
• Replicon: chromosome
• Accession: NZ_CP035382
• Organism: Leclercia adecarboxylata strain R25

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: ES815_RS21355
• Protein ID: WP_142489621.1
• Coordinates: 4278146..4278574 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A345CHX5
• Locus tag: ES815_RS21360
• Protein ID: WP_039032651.1
• Coordinates: 4278571..4278801 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ES815_RS21335	4274194..4274583	-	390	WP_185902425.1	copper-binding protein	-
ES815_RS21340	4274666..4275574	-	909	WP_142489619.1	LysR family transcriptional regulator	-
ES815_RS21345	4275707..4277026	+	1320	WP_142489620.1	6-phospho-beta-glucosidase	-
ES815_RS21350	4277415..4278112	-	698	Protein_3926	IS1 family transposase	-
ES815_RS21355	4278146..4278574	-	429	WP_142489621.1	type II toxin-antitoxin system VapC family toxin	Toxin
ES815_RS21360	4278571..4278801	-	231	WP_039032651.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ES815_RS21365	4279223..4280488	-	1266	WP_142489622.1	NO-inducible flavohemoprotein	-
ES815_RS21370	4280723..4281025	+	303	WP_185902359.1	hypothetical protein	-
ES815_RS21375	4281840..4282241	-	402	WP_142489624.1	H-NS histone family protein	-
ES815_RS21380	4282385..4282615	+	231	WP_142489625.1	hypothetical protein	-
ES815_RS21385	4282838..4283535	+	698	Protein_3933	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4274666..4314526	39860
	-	inside	IScluster/Tn	-	-	4277415..4289339	11924

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15554.05 Da
• Isoelectric Point: 5.7261

>T117685 WP_142489621.1 NZ_CP035382:c4278574-4278146 [Leclercia adecarboxylata]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRNQRIVISAITYSEMRFGATGPKSSPRHIELVDAFCARLDAILPWDSA
AVDATTDIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVMLPTY

Nucleotide

• Length: 429 bp

>T117685 NZ_CP035382:c4278574-4278146 [Leclercia adecarboxylata]
GTGAACAAAACCTATATGCTGGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTACTGAAGCGCCT
GGAGCAGGCGGTGCTGCGAAACCAGCGCATCGTGATCTCGGCCATTACCTATTCCGAGATGCGCTTTGGCGCCACCGGTC
CGAAGTCCTCGCCGCGCCACATCGAGCTGGTTGACGCGTTTTGCGCCCGCCTCGATGCCATCCTGCCCTGGGACAGCGCC
GCGGTGGATGCGACCACGGACATAAAGGTGGCGCTGCGCCTCGCAGGGACGCCGATCGGCCCGAACGACACGGCGATCGC
CGGGCATGCCATTGCCGCCGGCGCCGTGCTGGTGACGAATAATGTGCGGGAGTTTGAGCGGGTGCCGGGCCTGGTGCTGG
AAGACTGGGTGATGCTGCCAACTTACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8737.83 Da
• Isoelectric Point: 4.3654

>AT117685 WP_039032651.1 NZ_CP035382:c4278801-4278571 [Leclercia adecarboxylata]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVTDEGRFDL

Nucleotide

• Length: 231 bp

>AT117685 NZ_CP035382:c4278801-4278571 [Leclercia adecarboxylata]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTCG
AGAAGGCCGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCACCGACGAAGGACGATTTGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A345CHX5