Detailed information of TA system TA117683

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2929108..2929727
• Replicon: chromosome
• Accession: NZ_CP035382
• Organism: Leclercia adecarboxylata strain R25

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A345CDT0
• Locus tag: ES815_RS14905
• Protein ID: WP_032616864.1
• Coordinates: 2929108..2929326 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: ES815_RS14910
• Protein ID: WP_142488463.1
• Coordinates: 2929335..2929727 (-)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ES815_RS14885	2926021..2926374	+	354	WP_142488459.1	DUF1428 family protein	-
ES815_RS14890	2926407..2927975	-	1569	WP_142488460.1	EAL domain-containing protein	-
ES815_RS14895	2928169..2928309	+	141	WP_142488461.1	type B 50S ribosomal protein L36	-
ES815_RS14900	2928369..2928833	-	465	WP_142488462.1	hypothetical protein	-
ES815_RS14905	2929108..2929326	-	219	WP_032616864.1	hemolysin expression modulator Hha	Toxin
ES815_RS14910	2929335..2929727	-	393	WP_142488463.1	Hha toxicity modulator TomB	Antitoxin
ES815_RS14915	2930220..2933360	-	3141	WP_142488464.1	multidrug efflux RND transporter permease subunit AcrB	-
ES815_RS14920	2933383..2934576	-	1194	WP_142488465.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-115)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8583.95 Da
• Isoelectric Point: 8.9008

>T117683 WP_032616864.1 NZ_CP035382:c2929326-2929108 [Leclercia adecarboxylata]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTAVWKFVR

Nucleotide

• Length: 219 bp

>T117683 NZ_CP035382:c2929326-2929108 [Leclercia adecarboxylata]
ATGTCTGATAAACCATTAACCAAGACAGATTATTTGATGCGTTTGCGACGCTGTCAGTCAATTGACACCCTTGAACGTGT
GATTGAGAAAAATAAATACGAGCTTTCTGATAATGAGTTAGCGGTATTCTATTCCGCAGCGGATCATCGCCTGGCTGAAC
TTACGATGAATAAGCTGTACGATAAAATTCCTACCGCCGTGTGGAAATTCGTACGCTGA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 15122.00 Da
• Isoelectric Point: 4.8941

>AT117683 WP_142488463.1 NZ_CP035382:c2929727-2929335 [Leclercia adecarboxylata]
MDEYSPKRQDIAQLKFLCESLYHDCLSNLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNASRANPLASPVKIITNN

Nucleotide

• Length: 393 bp

>AT117683 NZ_CP035382:c2929727-2929335 [Leclercia adecarboxylata]
ATGGACGAATACTCGCCCAAAAGGCAAGATATTGCACAGTTGAAATTCCTTTGTGAATCCCTGTACCATGACTGCCTTTC
CAATCTCGATGAAAGCAACCATGGCTGGGTCAATGACCCTACTTCTGCCATTAATTTGCAGCTAAACGAGTTGATTGAGC
ATATAGCAACCTTCGCCCTTAATTATAAAATTAAGTATAACGAAGATAATAAGTTGATTGAGCAAATTGACGAATACCTG
GATGACACTTTTATGTTATTCAGCAGCTACGGCATTAACGCGCAGGATTTGCAGAAATGGCGTAAATCGGGCAATCGGCT
TTTTCGTTGTTTTGTCAATGCAAGCAGGGCTAACCCGTTAGCTTCTCCGGTTAAAATTATTACAAACAATTAG

Structures

Toxin

• AlphaFold DB: A0A345CDT0