Detailed information of TA system TA117681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): KacT-ataR/DUF1778(antitoxin)
• Location: 2425825..2426619
• Replicon: chromosome
• Accession: NZ_CP035382
• Organism: Leclercia adecarboxylata strain R25

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: ES815_RS12540
• Protein ID: WP_142490051.1
• Coordinates: 2425825..2426325 (-)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: ES815_RS12545
• Protein ID: WP_142488082.1
• Coordinates: 2426350..2426619 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ES815_RS12515	2420991..2421245	-	255	WP_142488077.1	DUF4282 domain-containing protein	-
ES815_RS12520	2421360..2422751	-	1392	WP_142488078.1	PLP-dependent aminotransferase family protein	-
ES815_RS12525	2422852..2423331	+	480	WP_142488079.1	carboxymuconolactone decarboxylase family protein	-
ES815_RS12530	2423318..2424220	-	903	WP_142488080.1	LysR family transcriptional regulator	-
ES815_RS12535	2424321..2425736	+	1416	WP_142488081.1	aldehyde dehydrogenase family protein	-
ES815_RS12540	2425825..2426325	-	501	WP_142490051.1	GNAT family N-acetyltransferase	Toxin
ES815_RS12545	2426350..2426619	-	270	WP_142488082.1	DUF1778 domain-containing protein	Antitoxin
ES815_RS12550	2426782..2427105	+	324	WP_142488083.1	antibiotic biosynthesis monooxygenase	-
ES815_RS12555	2427098..2427493	+	396	WP_142488084.1	amino acid-binding protein	-
ES815_RS12560	2427468..2428163	+	696	WP_142488085.1	winged helix-turn-helix domain-containing protein	-
ES815_RS12565	2428459..2428848	+	390	WP_142488086.1	hypothetical protein	-
ES815_RS12570	2429156..2429719	+	564	WP_185902332.1	helix-turn-helix transcriptional regulator	-
ES815_RS12575	2429737..2430078	+	342	WP_142488088.1	hypothetical protein	-
ES815_RS12580	2430194..2430661	+	468	WP_142488089.1	MarR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (78-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18559.34 Da
• Isoelectric Point: 7.3982

>T117681 WP_142490051.1 NZ_CP035382:c2426325-2425825 [Leclercia adecarboxylata]
MFSQTTHYDLTGFNCGEDTLNRFLCEHLARQHVGRLLRAYLLVTDDPTPKVLGYYTLSGSCFEKETLPSKTQQRKVPYVN
VPSVTLGRLAIHQDLQGQGWGMTLVTHAMKVVWLASQAVGIHGMFVDALNDKAKDFYLTLGFIPLTGSNADALFYPTKSI
EKLFAE

Nucleotide

• Length: 501 bp

>T117681 NZ_CP035382:c2426325-2425825 [Leclercia adecarboxylata]
ATGTTTTCGCAAACGACTCACTATGATTTAACAGGCTTCAACTGCGGGGAAGATACCCTCAACCGTTTTCTGTGTGAGCA
TCTTGCACGCCAGCACGTTGGGCGCTTGTTACGCGCTTATCTTCTCGTCACCGATGACCCTACGCCTAAAGTGCTGGGCT
ACTATACGTTGTCCGGCAGTTGCTTTGAGAAAGAGACCCTGCCATCAAAAACGCAGCAGCGCAAAGTGCCCTATGTCAAT
GTACCCAGCGTCACGTTAGGGCGCCTCGCAATACACCAGGATCTGCAGGGTCAGGGCTGGGGGATGACGCTGGTGACACA
TGCCATGAAAGTGGTCTGGCTGGCGTCGCAGGCTGTGGGCATTCACGGCATGTTTGTGGATGCGCTCAACGATAAGGCGA
AAGATTTTTATCTGACGCTCGGCTTTATTCCTTTAACGGGTAGCAACGCCGATGCTCTTTTTTATCCCACCAAATCCATT
GAAAAGCTGTTTGCCGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10415.85 Da
• Isoelectric Point: 5.2171

>AT117681 WP_142488082.1 NZ_CP035382:c2426619-2426350 [Leclercia adecarboxylata]
MPALKKQRIDLRLTDEDKDLIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLILNEDSWKRVMDALDNPPEPNERLKR
AARRLQNME

Nucleotide

• Length: 270 bp

>AT117681 NZ_CP035382:c2426619-2426350 [Leclercia adecarboxylata]
ATGCCAGCACTGAAAAAACAGCGCATCGACCTCAGGTTAACTGACGAAGACAAAGATCTGATCGAAGAAGCTGCGGCGAT
GACTAACCAGACAATCACCCAGTTTATGGTTAACAGTGCCTCGGAACGGGCTGCCGAAGTCATTGAGCAGCATCGCCGCC
TGATCCTTAATGAAGATTCGTGGAAACGCGTTATGGATGCACTCGACAACCCGCCAGAGCCCAATGAGAGGCTGAAACGC
GCAGCAAGACGTCTGCAAAACATGGAGTAA