Detailed information of TA system TA117677

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 1542157..1542743
• Replicon: chromosome
• Accession: NZ_CP035382
• Organism: Leclercia adecarboxylata strain R25

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: ES815_RS08370
• Protein ID: WP_142487420.1
• Coordinates: 1542157..1542525 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: ES815_RS08375
• Protein ID: WP_142487421.1
• Coordinates: 1542522..1542743 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ES815_RS08350	1537786..1538856	-	1071	WP_142487416.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-
ES815_RS08355	1538858..1539703	-	846	WP_142487417.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
ES815_RS08360	1539700..1540587	-	888	WP_142487418.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
ES815_RS08365	1540688..1542004	-	1317	WP_142487419.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
ES815_RS08370	1542157..1542525	-	369	WP_142487420.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
ES815_RS08375	1542522..1542743	-	222	WP_142487421.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ES815_RS08380	1542891..1543604	-	714	WP_072250910.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
ES815_RS08385	1543622..1544389	-	768	WP_142487422.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
ES815_RS08390	1544386..1545663	-	1278	WP_142487423.1	high-affinity branched-chain amino acid ABC transporter permease LivM	-
ES815_RS08395	1545660..1546586	-	927	WP_039032510.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1537049..1544389	7340

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (29-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13645.94 Da
• Isoelectric Point: 8.4948

>T117677 WP_142487420.1 NZ_CP035382:c1542525-1542157 [Leclercia adecarboxylata]
MTLQFISAEEIVLFHDKLLSVTPGVAGMSDPGRAEALLYRVLNKYQYEGVTDVWILAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGISLKANPDYVAMTVEAAAGRLSLEEIVERLRR

Nucleotide

• Length: 369 bp

>T117677 NZ_CP035382:c1542525-1542157 [Leclercia adecarboxylata]
ATGACCCTGCAATTTATCTCAGCGGAAGAGATAGTCCTGTTTCATGACAAACTCCTCAGCGTCACGCCTGGCGTCGCGGG
AATGTCGGATCCGGGTCGTGCTGAAGCGCTGCTCTATCGCGTGTTAAACAAATATCAATACGAAGGGGTGACCGATGTCT
GGATCCTGGCGGCGATGCATTTGCTGGCCATCTCCCGCGGCCATATCTTCAATGATGGTAATAAACGCACGGCATTGTTT
ATCACCTTGCTTTTCCTCAAACGTAACGGCATTTCGCTGAAGGCCAATCCGGATTATGTGGCGATGACGGTTGAGGCTGC
TGCGGGCCGTCTGTCGCTTGAAGAAATTGTGGAGCGTTTACGCCGCTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8078.10 Da
• Isoelectric Point: 5.6579

>AT117677 WP_142487421.1 NZ_CP035382:c1542743-1542522 [Leclercia adecarboxylata]
MRTMNYSEARQNLASALDSAATGTPVTITRRGHKPAVIISAEEFERYQVAKLDAEFDAIMGIHGNEIRELADK

Nucleotide

• Length: 222 bp

>AT117677 NZ_CP035382:c1542743-1542522 [Leclercia adecarboxylata]
ATGCGTACAATGAACTATAGCGAAGCACGTCAGAATCTTGCCTCAGCGCTGGATTCTGCCGCGACGGGAACACCCGTCAC
CATCACGCGTCGCGGTCATAAACCTGCCGTGATTATCAGCGCAGAAGAATTTGAACGTTATCAGGTGGCAAAACTGGATG
CCGAGTTTGACGCCATTATGGGTATTCATGGCAACGAAATTAGGGAACTGGCTGACAAATGA