Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 21024..21667 | Replicon | plasmid pLA-109 |
| Accession | NZ_CP035380 | ||
| Organism | Leclercia adecarboxylata strain R25 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | A0A6C0NGR2 |
| Locus tag | ES815_RS00120 | Protein ID | WP_008322222.1 |
| Coordinates | 21251..21667 (+) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | A0A6C0NE67 |
| Locus tag | ES815_RS00115 | Protein ID | WP_008322219.1 |
| Coordinates | 21024..21254 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ES815_RS00080 | 16165..17094 | + | 930 | WP_000998778.1 | copper resistance inner membrane protein PcoD | - |
| ES815_RS00085 | 17149..17829 | + | 681 | WP_103181204.1 | copper response regulator transcription factor PcoR | - |
| ES815_RS00090 | 17826..19226 | + | 1401 | WP_103825201.1 | copper resistance membrane spanning protein PcoS | - |
| ES815_RS00095 | 19442..19876 | + | 435 | WP_004388336.1 | copper resistance system metallochaperone PcoE | - |
| ES815_RS00100 | 20108..20404 | - | 297 | WP_045343599.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| ES815_RS00105 | 20391..20651 | - | 261 | WP_040219223.1 | type II toxin-antitoxin system ParD family antitoxin | - |
| ES815_RS00115 | 21024..21254 | + | 231 | WP_008322219.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | Antitoxin |
| ES815_RS00120 | 21251..21667 | + | 417 | WP_008322222.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| ES815_RS00125 | 21741..23303 | + | 1563 | WP_008322224.1 | AAA family ATPase | - |
| ES815_RS00130 | 23288..24310 | + | 1023 | WP_008322227.1 | helicase UvrD | - |
| ES815_RS23680 | 24354..24494 | + | 141 | WP_158250576.1 | hypothetical protein | - |
| ES815_RS00135 | 24632..24937 | + | 306 | WP_103825202.1 | metalloregulator ArsR/SmtB family transcription factor | - |
| ES815_RS00140 | 24954..25667 | + | 714 | WP_181247314.1 | arsenical resistance protein ArsH | - |
| ES815_RS00145 | 25676..26221 | + | 546 | WP_103825203.1 | sigma-70 family RNA polymerase sigma factor | - |
| ES815_RS00150 | 26297..26659 | + | 363 | WP_103825204.1 | arsenic metallochaperone ArsD family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..108995 | 108995 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15188.56 Da Isoelectric Point: 7.1361
>T117672 WP_008322222.1 NZ_CP035380:21251-21667 [Leclercia adecarboxylata]
VNKTYMLDTNICSFIMREQPEAVLKRLEQAVLRNQRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
VNKTYMLDTNICSFIMREQPEAVLKRLEQAVLRNQRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
Download Length: 417 bp
>T117672 NZ_CP035380:21251-21667 [Leclercia adecarboxylata]
GTGAACAAGACCTATATGCTGGACACCAACATCTGCTCATTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
TGAGCAGGCGGTGCTGCGTAACCAGCGCATCGTTGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCACATCGAGCTGGTTGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCGCTGCGCATGGCCGGCACGCCGATCGGCCCGAACGACACGGCTATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTAGTGCTGG
AAGACTGGGTAAGATAA
GTGAACAAGACCTATATGCTGGACACCAACATCTGCTCATTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
TGAGCAGGCGGTGCTGCGTAACCAGCGCATCGTTGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCACATCGAGCTGGTTGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCGCTGCGCATGGCCGGCACGCCGATCGGCCCGAACGACACGGCTATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTAGTGCTGG
AAGACTGGGTAAGATAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8736.84 Da Isoelectric Point: 4.4692
>AT117672 WP_008322219.1 NZ_CP035380:21024-21254 [Leclercia adecarboxylata]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVTDEGRFNL
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVTDEGRFNL
Download Length: 231 bp
>AT117672 NZ_CP035380:21024-21254 [Leclercia adecarboxylata]
ATGAGAACCGTATCTATTTTCAAAAATGGCAACAACCGCGCCATCCGCCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTCG
AAAAAGCTGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCACCGACGAAGGACGATTTAACCTGTGA
ATGAGAACCGTATCTATTTTCAAAAATGGCAACAACCGCGCCATCCGCCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTCG
AAAAAGCTGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCACCGACGAAGGACGATTTAACCTGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A6C0NGR2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A6C0NE67 |