Detailed information of TA system TA117670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2796089..2796618
• Replicon: chromosome
• Accession: NZ_CP035369
• Organism: Staphylococcus aureus strain LAC

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ERW10_RS14645
• Protein ID: WP_000621175.1
• Coordinates: 2796256..2796618 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: ERW10_RS14640
• Protein ID: WP_000948331.1
• Coordinates: 2796089..2796259 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ERW10_RS14610	2791126..2791686	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
ERW10_RS14615	2791894..2792373	+	480	WP_001287088.1	hypothetical protein	-
ERW10_RS14620	2792366..2793949	+	1584	WP_001294626.1	PH domain-containing protein	-
ERW10_RS14625	2793936..2794427	+	492	WP_001205910.1	PH domain-containing protein	-
ERW10_RS14630	2794431..2794790	+	360	WP_000581200.1	holo-ACP synthase	-
ERW10_RS14635	2794856..2796004	+	1149	WP_001281145.1	alanine racemase	-
ERW10_RS14640	2796089..2796259	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
ERW10_RS14645	2796256..2796618	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ERW10_RS14655	2796968..2797969	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
ERW10_RS14660	2798088..2798414	+	327	WP_001052491.1	anti-sigma factor antagonist	-
ERW10_RS14665	2798416..2798895	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
ERW10_RS14670	2798870..2799640	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T117670 WP_000621175.1 NZ_CP035369:2796256-2796618 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T117670 NZ_CP035369:2796256-2796618 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT117670 WP_000948331.1 NZ_CP035369:2796089-2796259 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT117670 NZ_CP035369:2796089-2796259 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1