Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4337275..4337906 Replicon chromosome
Accession NZ_CP035366
Organism Escherichia coli O157:H7 strain C1-057

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag ES669_RS22520 Protein ID WP_001259385.1
Coordinates 4337631..4337906 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag ES669_RS22515 Protein ID WP_000593555.1
Coordinates 4337275..4337634 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ES669_RS22485 4332387..4332860 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
ES669_RS22490 4332857..4333138 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
ES669_RS22495 4333215..4334573 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
ES669_RS22500 4334566..4336074 + 1509 WP_001301773.1 carbohydrate kinase -
ES669_RS22505 4336064..4336333 + 270 WP_000933060.1 HPr family phosphocarrier protein -
ES669_RS22510 4336365..4337225 + 861 WP_001131788.1 class II aldolase -
ES669_RS22515 4337275..4337634 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ES669_RS22520 4337631..4337906 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
ES669_RS22525 4337979..4339103 - 1125 WP_001301659.1 ABC transporter permease -
ES669_RS22530 4339103..4341838 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
ES669_RS22535 4341835..4342902 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T117645 WP_001259385.1 NZ_CP035366:c4337906-4337631 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T117645 NZ_CP035366:c4337906-4337631 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT117645 WP_000593555.1 NZ_CP035366:c4337634-4337275 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT117645 NZ_CP035366:c4337634-4337275 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References