Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 572084..572702 Replicon chromosome
Accession NZ_CP035366
Organism Escherichia coli O157:H7 strain C1-057

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ES669_RS02790 Protein ID WP_001291435.1
Coordinates 572084..572302 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ES669_RS02795 Protein ID WP_000344800.1
Coordinates 572328..572702 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ES669_RS02755 567371..567943 + 573 WP_000779831.1 YbaY family lipoprotein -
ES669_RS02760 567976..568287 - 312 WP_000409911.1 MGMT family protein -
ES669_RS02770 568666..569019 + 354 WP_000878140.1 DUF1428 domain-containing protein -
ES669_RS02775 569061..570611 - 1551 WP_001302952.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ES669_RS02780 570775..571245 - 471 WP_000136192.1 YlaC family protein -
ES669_RS02785 571361..571912 - 552 WP_000102553.1 maltose O-acetyltransferase -
ES669_RS02790 572084..572302 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
ES669_RS02795 572328..572702 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ES669_RS02800 573248..576397 - 3150 WP_046671444.1 efflux RND transporter permease AcrB -
ES669_RS02805 576420..577613 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T117616 WP_001291435.1 NZ_CP035366:c572302-572084 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T117616 NZ_CP035366:c572302-572084 [Escherichia coli O157:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT117616 WP_000344800.1 NZ_CP035366:c572702-572328 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT117616 NZ_CP035366:c572702-572328 [Escherichia coli O157:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References