Detailed information of TA system TA117610

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 27066..27588
• Replicon: plasmid pD72-IncX1
• Accession: NZ_CP035315
• Organism: Escherichia coli strain D72

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G3CAN5
• Locus tag: ERL60_RS23635
• Protein ID: WP_000220560.1
• Coordinates: 27066..27347 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: ERL60_RS23640
• Protein ID: WP_000121743.1
• Coordinates: 27337..27588 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ERL60_RS23595	22075..22566	-	492	WP_000872475.1	transcription termination factor NusG	-
ERL60_RS23600	22927..24084	-	1158	WP_000538023.1	hypothetical protein	-
ERL60_RS23605	24087..24632	-	546	WP_038976855.1	hypothetical protein	-
ERL60_RS23610	24959..25231	-	273	WP_000160399.1	hypothetical protein	-
ERL60_RS24010	25244..25393	-	150	WP_000003880.1	hypothetical protein	-
ERL60_RS23615	25680..26009	-	330	WP_000866648.1	hypothetical protein	-
ERL60_RS23620	26102..26317	-	216	WP_001180116.1	hypothetical protein	-
ERL60_RS23625	26307..26552	-	246	WP_000356546.1	hypothetical protein	-
ERL60_RS23630	26597..26920	-	324	WP_001181903.1	hypothetical protein	-
ERL60_RS23635	27066..27347	-	282	WP_000220560.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ERL60_RS23640	27337..27588	-	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
ERL60_RS23660	28844..29680	+	837	WP_001050931.1	replication initiation protein	-
ERL60_RS23665	29720..30166	+	447	WP_001074386.1	hypothetical protein	-
ERL60_RS23670	30234..30656	+	423	WP_031942371.1	DNA distortion polypeptide 3	-
ERL60_RS23675	30716..31684	-	969	WP_077249520.1	IS5-like element IS903B family transposase	-
ERL60_RS23680	31686..31979	-	294	WP_015060510.1	hypothetical protein	-
ERL60_RS23685	31995..32225	-	231	WP_000051064.1	partitioning protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrS2 / aph(3')-Ia	-	1..40654	40654

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11005.83 Da
• Isoelectric Point: 10.5938

>T117610 WP_000220560.1 NZ_CP035315:c27347-27066 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Nucleotide

• Length: 282 bp

>T117610 NZ_CP035315:c27347-27066 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT117610 WP_000121743.1 NZ_CP035315:c27588-27337 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT117610 NZ_CP035315:c27588-27337 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: G3CAN5

Antitoxin

• AlphaFold DB: A0A3S5I301