Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 77385..78028 Replicon plasmid pD72-F33
Accession NZ_CP035314
Organism Escherichia coli strain D72

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag ERL60_RS23380 Protein ID WP_001044768.1
Coordinates 77612..78028 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag ERL60_RS23375 Protein ID WP_001261287.1
Coordinates 77385..77615 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ERL60_RS23330 73068..73325 + 258 WP_000557619.1 type II toxin-antitoxin system antitoxin PemI -
ERL60_RS23335 73258..73659 + 402 WP_001398199.1 type II toxin-antitoxin system toxin endoribonuclease PemK -
ERL60_RS23340 73908..74323 + 416 Protein_91 IS1 family transposase -
ERL60_RS23345 74359..74904 - 546 Protein_92 Tn3 family transposase -
ERL60_RS24005 74970..75062 - 93 Protein_93 IS6 family transposase -
ERL60_RS23355 75228..76427 - 1200 WP_000948429.1 IS91 family transposase -
ERL60_RS23360 76437..76625 - 189 WP_000957857.1 hypothetical protein -
ERL60_RS23365 76799..77089 - 291 WP_000111771.1 hypothetical protein -
ERL60_RS23375 77385..77615 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
ERL60_RS23380 77612..78028 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
ERL60_RS23385 78190..80328 - 2139 WP_000350638.1 AAA family ATPase -
ERL60_RS23390 80682..80939 + 258 WP_000343085.1 hypothetical protein -
ERL60_RS23395 80939..81529 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / blaCTX-M-55 - 1..89963 89963


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T117609 WP_001044768.1 NZ_CP035314:77612-78028 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T117609 NZ_CP035314:77612-78028 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT117609 WP_001261287.1 NZ_CP035314:77385-77615 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT117609 NZ_CP035314:77385-77615 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References