Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2395183..2395821 Replicon chromosome
Accession NZ_CP035312
Organism Escherichia coli strain D72

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag ERL60_RS11650 Protein ID WP_000813794.1
Coordinates 2395645..2395821 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag ERL60_RS11645 Protein ID WP_001270286.1
Coordinates 2395183..2395599 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ERL60_RS11625 2390335..2391276 - 942 WP_001251319.1 ABC transporter permease -
ERL60_RS11630 2391277..2392290 - 1014 WP_131419357.1 ABC transporter ATP-binding protein -
ERL60_RS11635 2392308..2393453 - 1146 Protein_2246 ABC transporter substrate-binding protein -
ERL60_RS11640 2393695..2395104 - 1410 WP_000760625.1 PLP-dependent aminotransferase family protein -
ERL60_RS11645 2395183..2395599 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
ERL60_RS11650 2395645..2395821 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
ERL60_RS11655 2396043..2396273 + 231 WP_000494244.1 YncJ family protein -
ERL60_RS11660 2396365..2398326 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
ERL60_RS11665 2398399..2398935 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
ERL60_RS11670 2398988..2400202 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T117595 WP_000813794.1 NZ_CP035312:c2395821-2395645 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T117595 NZ_CP035312:c2395821-2395645 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT117595 WP_001270286.1 NZ_CP035312:c2395599-2395183 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT117595 NZ_CP035312:c2395599-2395183 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References