Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1933216..1933707 Replicon chromosome
Accession NZ_CP035309
Organism Macrococcus canis strain KM0218

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag EST43_RS10015 Protein ID WP_138072443.1
Coordinates 1933216..1933566 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag EST43_RS10020 Protein ID WP_138072445.1
Coordinates 1933567..1933707 (-) Length 47 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EST43_RS09995 1930536..1931303 - 768 WP_133420462.1 RNA polymerase sigma factor SigB -
EST43_RS10000 1931308..1931751 - 444 WP_138072439.1 anti-sigma B factor RsbW -
EST43_RS10005 1931756..1932079 - 324 WP_086043353.1 anti-sigma factor antagonist -
EST43_RS10010 1932160..1933167 - 1008 WP_138072441.1 PP2C family protein-serine/threonine phosphatase -
EST43_RS10015 1933216..1933566 - 351 WP_138072443.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EST43_RS10020 1933567..1933707 - 141 WP_138072445.1 antitoxin MazE Antitoxin
EST43_RS10025 1933769..1934911 - 1143 WP_138072447.1 alanine racemase -
EST43_RS10030 1934937..1935293 - 357 WP_138072449.1 holo-ACP synthase -
EST43_RS10035 1935235..1935756 - 522 WP_138072451.1 PH domain-containing protein -
EST43_RS10040 1935740..1937251 - 1512 WP_138072453.1 PH domain-containing protein -
EST43_RS10045 1937241..1937717 - 477 WP_138072455.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12750.96 Da        Isoelectric Point: 9.7528

>T117580 WP_138072443.1 NZ_CP035309:c1933566-1933216 [Macrococcus canis]
MKRGDVYLADLSPVKGSEQGGKRPVVIIQNNIGNKHSPTVIVAAITAKINKAKIPTHVEIGKTAYHLEKDSVILLEQIRT
IDKYRLIDHLTVLDKAKMKEVDVALLISLGLSIDSE

Download         Length: 351 bp

>T117580 NZ_CP035309:c1933566-1933216 [Macrococcus canis]
ATGAAACGTGGAGATGTTTATTTAGCTGATCTGTCACCAGTGAAGGGGTCTGAACAAGGCGGTAAACGCCCCGTCGTTAT
TATTCAGAATAATATTGGGAATAAACATAGTCCTACAGTGATTGTAGCCGCAATTACAGCAAAGATTAATAAAGCAAAGA
TCCCCACCCATGTGGAAATTGGAAAGACCGCGTATCACCTGGAAAAGGATTCGGTAATATTGCTTGAACAAATTAGAACT
ATTGATAAGTATAGGTTAATTGATCATCTGACAGTGCTTGATAAGGCAAAGATGAAAGAAGTGGATGTGGCACTTTTAAT
TAGTTTAGGGTTATCAATCGATAGTGAATAG

Antitoxin


Download         Length: 47 a.a.        Molecular weight: 5319.89 Da        Isoelectric Point: 3.8301

>AT117580 WP_138072445.1 NZ_CP035309:c1933707-1933567 [Macrococcus canis]
MSQKLEQMLIEGYQSMAEINLSIASEHYILECEVNDNHQTYTIDGE

Download         Length: 141 bp

>AT117580 NZ_CP035309:c1933707-1933567 [Macrococcus canis]
ATGAGCCAGAAGCTGGAGCAAATGCTGATTGAAGGATATCAATCAATGGCAGAGATTAATTTATCGATAGCATCAGAACA
TTATATATTAGAATGTGAAGTTAATGATAACCATCAGACATATACCATTGATGGTGAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References