Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 2533923..2534556 Replicon chromosome
Accession NZ_CP035305
Organism Weizmannia coagulans strain IDCC1201

Toxin (Protein)


Gene name mazF Uniprot ID G2TIM0
Locus tag ESP47_RS12465 Protein ID WP_013860680.1
Coordinates 2533923..2534273 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID G2TIL9
Locus tag ESP47_RS12470 Protein ID WP_014096808.1
Coordinates 2534278..2534556 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ESP47_RS12425 2529181..2529960 - 780 WP_014096815.1 RNA polymerase sigma factor SigB -
ESP47_RS12430 2529938..2530408 - 471 WP_014096814.1 anti-sigma B factor RsbW -
ESP47_RS12435 2530412..2530747 - 336 WP_026104587.1 anti-sigma factor antagonist -
ESP47_RS12440 2530817..2531826 - 1010 Protein_2358 PP2C family protein-serine/threonine phosphatase -
ESP47_RS12445 2531840..2532241 - 402 WP_014096811.1 anti-sigma regulatory factor -
ESP47_RS12450 2532246..2532602 - 357 WP_014096810.1 STAS domain-containing protein -
ESP47_RS12455 2532606..2533433 - 828 WP_014096809.1 RsbT co-antagonist protein RsbRA -
ESP47_RS12465 2533923..2534273 - 351 WP_013860680.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ESP47_RS12470 2534278..2534556 - 279 WP_014096808.1 hypothetical protein Antitoxin
ESP47_RS12475 2534709..2535860 - 1152 WP_014096807.1 alanine racemase -
ESP47_RS12480 2536062..2537078 - 1017 WP_035183268.1 outer membrane lipoprotein carrier protein LolA -
ESP47_RS12485 2537219..2537569 - 351 WP_014096805.1 holo-ACP synthase -
ESP47_RS12490 2537850..2538449 + 600 WP_014096804.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12968.01 Da        Isoelectric Point: 4.8998

>T117577 WP_013860680.1 NZ_CP035305:c2534273-2533923 [Weizmannia coagulans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGLERDSVILLEQI
RTIDKQRLTDKITHLDEEVMEKIDDALQISLGLVEF

Download         Length: 351 bp

>T117577 NZ_CP035305:c2534273-2533923 [Weizmannia coagulans]
TTGATTGTTAAGCGTGGAGACGTGTACTTCGCCGATCTATCTCCGGTGGTCGGCTCCGAGCAGGGAGGCGTACGTCCTGT
CCTCATTTTACAAAACGACATTGGAAACCGTTTTAGCCCCACTGTCATTGTTGCCGCGATAACAGCGCAAATCCAGAAAG
CAAAATTGCCCACGCATGTGGAAATTGATGCAAAGCGTTACGGTTTGGAAAGGGATTCTGTGATATTGCTTGAACAAATA
CGCACAATCGATAAACAACGCTTAACGGATAAGATTACGCATCTTGATGAAGAAGTCATGGAAAAAATTGACGACGCCCT
GCAAATCAGCCTGGGACTGGTCGAATTTTAG

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10633.19 Da        Isoelectric Point: 5.2204

>AT117577 WP_014096808.1 NZ_CP035305:c2534556-2534278 [Weizmannia coagulans]
VSEPISTEILVRLPQQLLTELDGFAETENMNRNELIYRATKMYLKERKKRQIRETMRRGYMEMAKINLAIASEAIQAEYE
AEHTVESLVSGG

Download         Length: 279 bp

>AT117577 NZ_CP035305:c2534556-2534278 [Weizmannia coagulans]
GTGTCTGAACCTATTTCAACAGAAATTTTAGTCCGCTTGCCGCAGCAGCTGTTGACGGAATTGGACGGATTTGCTGAAAC
TGAAAATATGAATCGCAATGAATTAATTTACCGTGCCACAAAAATGTATTTAAAAGAACGGAAAAAACGCCAAATCCGCG
AAACGATGAGGCGTGGTTACATGGAAATGGCAAAAATTAATTTGGCGATTGCCTCCGAAGCGATCCAGGCTGAATACGAA
GCAGAACATACAGTTGAAAGCCTAGTAAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4BYJ0

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4BX66

References