Detailed information of TA system TA117574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 3218216..3218796
• Replicon: chromosome
• Accession: NZ_CP035300
• Organism: Dyella sp. M7H15-1

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: EO087_RS14750
• Protein ID: WP_128899527.1
• Coordinates: 3218470..3218796 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: EO087_RS14745
• Protein ID: WP_128899526.1
• Coordinates: 3218216..3218473 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EO087_RS14730	3215328..3215990	+	663	WP_128899525.1	outer membrane lipoprotein chaperone LolA	-
EO087_RS14735	3216001..3216559	+	559	Protein_2912	DUF1275 domain-containing protein	-
EO087_RS14740	3216607..3217962	+	1356	WP_128899979.1	replication-associated recombination protein A	-
EO087_RS14745	3218216..3218473	+	258	WP_128899526.1	antitoxin	Antitoxin
EO087_RS14750	3218470..3218796	+	327	WP_128899527.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EO087_RS14755	3218901..3220181	+	1281	WP_128899528.1	serine--tRNA ligase	-
EO087_RS14760	3220178..3220555	+	378	WP_128899529.1	LrgA	-
EO087_RS14770	3221381..3222139	-	759	WP_128897459.1	IS21-like element helper ATPase IstB	-
EO087_RS14775	3222153..3223721	-	1569	WP_128898324.1	IS21 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3221381..3222139	758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-107)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11491.39 Da
• Isoelectric Point: 7.8322

>T117574 WP_128899527.1 NZ_CP035300:3218470-3218796 [Dyella sp. M7H15-1]
MNRGDIYLVSLDPTASHEQSGSRPVLIVTPTAFNTTTKLPVVLPITNGGEFARRLGFAVPITGVKTTGIVRCDQPCVLDL
AARGGRKVDTLPVAIMDEVLAKVATLFE

Nucleotide

• Length: 327 bp

>T117574 NZ_CP035300:3218470-3218796 [Dyella sp. M7H15-1]
ATGAACCGTGGCGACATTTACCTGGTGTCGCTCGATCCGACGGCGAGCCACGAACAAAGCGGAAGCCGACCCGTGCTGAT
CGTGACTCCGACCGCATTCAACACAACCACTAAGTTGCCCGTAGTGCTGCCGATCACTAACGGTGGGGAGTTTGCGCGTC
GGCTCGGTTTTGCCGTGCCGATCACTGGCGTCAAAACCACCGGGATCGTGCGATGCGATCAACCGTGCGTGCTTGATCTC
GCTGCGCGGGGTGGGCGTAAGGTAGATACGCTGCCGGTGGCGATCATGGACGAAGTGCTGGCGAAGGTTGCCACGCTGTT
TGAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9191.45 Da
• Isoelectric Point: 4.4438

>AT117574 WP_128899526.1 NZ_CP035300:3218216-3218473 [Dyella sp. M7H15-1]
MYTTYLRKVGGSVMLAVPPAFLDQLHLQVGATVGLAVDHGRLVVEPNARPRYTLDELLAASDYSQPEAADNREWIDAPAV
GGELL

Nucleotide

• Length: 258 bp

>AT117574 NZ_CP035300:3218216-3218473 [Dyella sp. M7H15-1]
ATGTACACCACCTATCTGCGCAAAGTCGGCGGCTCGGTCATGCTGGCCGTCCCACCTGCTTTCCTTGATCAATTGCACCT
GCAAGTCGGCGCGACGGTCGGCTTGGCTGTGGACCACGGTCGCTTGGTGGTCGAACCGAACGCGCGGCCGCGCTACACCT
TGGATGAACTGCTGGCCGCCTCGGACTACAGCCAACCGGAAGCCGCCGATAATCGAGAGTGGATCGATGCGCCGGCCGTG
GGCGGCGAGCTGCTATGA