Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 946285..946910 Replicon chromosome
Accession NZ_CP035282
Organism Tissierellia sp. JN-28

Toxin (Protein)


Gene name mazF Uniprot ID A0A410QHF2
Locus tag EQM13_RS04525 Protein ID WP_071140359.1
Coordinates 946563..946910 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A410QA79
Locus tag EQM13_RS04520 Protein ID WP_071140314.1
Coordinates 946285..946566 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQM13_RS04495 942217..942828 + 612 WP_071140309.1 hypothetical protein -
EQM13_RS04500 942830..943084 + 255 WP_071140310.1 hypothetical protein -
EQM13_RS04505 943099..944364 + 1266 WP_071140311.1 NAD(P)H-hydrate dehydratase -
EQM13_RS04510 944370..944975 + 606 WP_071140312.1 hypothetical protein -
EQM13_RS04515 944991..946166 + 1176 WP_071140313.1 alanine racemase -
EQM13_RS04520 946285..946566 + 282 WP_071140314.1 ribbon-helix-helix protein, CopG family Antitoxin
EQM13_RS04525 946563..946910 + 348 WP_071140359.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EQM13_RS04530 947008..947460 + 453 WP_071140315.1 hypothetical protein -
EQM13_RS04535 947479..948543 + 1065 WP_071140316.1 GNAT family N-acetyltransferase -
EQM13_RS04540 948553..949485 + 933 WP_071140317.1 PIG-L family deacetylase -
EQM13_RS04545 949527..950657 + 1131 WP_083381899.1 glycosyltransferase family 4 protein -
EQM13_RS04550 950657..951148 + 492 WP_071140319.1 DUF4330 domain-containing protein -
EQM13_RS04555 951167..951724 + 558 WP_071140320.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin

Ribbon-helix-helix protein, CopG

(9-43)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12816.01 Da        Isoelectric Point: 7.9956

>T117538 WP_071140359.1 NZ_CP035282:946563-946910 [Tissierellia sp. JN-28]
VIIKRGDIFYADLSPVIGSEQGGVRPVLVVQNDIGNRYSPTIIIAAITSQINKAKLPTHVEINAEYGLPRDSVVLLEQIR
TIDKKRLREKIGRFSDEMMVKVDECIKISLGLVDL

Download         Length: 348 bp

>T117538 NZ_CP035282:946563-946910 [Tissierellia sp. JN-28]
GTGATTATAAAAAGAGGAGATATTTTTTATGCGGACTTAAGTCCGGTTATAGGTTCCGAACAAGGAGGGGTTCGGCCTGT
TTTAGTAGTTCAAAATGATATTGGGAATAGATATAGCCCTACAATTATTATAGCTGCGATTACATCTCAAATAAATAAGG
CTAAATTGCCTACACATGTGGAAATTAATGCGGAATATGGTCTTCCAAGAGATTCTGTAGTACTTTTAGAACAAATAAGA
ACTATAGATAAAAAAAGGCTTAGAGAAAAAATTGGAAGGTTCAGCGATGAAATGATGGTTAAGGTTGATGAGTGCATAAA
AATAAGTTTAGGGTTAGTGGACCTTTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10925.76 Da        Isoelectric Point: 4.9834

>AT117538 WP_071140314.1 NZ_CP035282:946285-946566 [Tissierellia sp. JN-28]
MAGTKKIMISLPDSLLEEVDFIVSIEKKNRSEFVREAMKLYIREKSKVEIEEKLKNGYKEMSKINLSIAEIGMEQDMREL
YVYEAKLTGCEQW

Download         Length: 282 bp

>AT117538 NZ_CP035282:946285-946566 [Tissierellia sp. JN-28]
ATGGCTGGGACTAAAAAAATTATGATAAGTTTACCTGATAGCTTATTGGAAGAAGTAGATTTTATAGTATCAATTGAAAA
AAAGAATAGAAGTGAATTTGTACGAGAGGCTATGAAACTGTACATTAGGGAAAAGAGCAAAGTGGAAATAGAAGAGAAGT
TAAAGAACGGATACAAAGAAATGAGTAAGATTAATTTGTCCATAGCTGAAATAGGTATGGAACAGGATATGAGAGAATTG
TATGTTTACGAAGCAAAATTAACAGGGTGTGAACAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A410QHF2

Antitoxin

Source ID Structure
AlphaFold DB A0A410QA79

References