Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4948616..4949232 Replicon chromosome
Accession NZ_CP035276
Organism Citrobacter freundii strain R17

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag EQ249_RS24260 Protein ID WP_003028682.1
Coordinates 4948616..4948990 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag EQ249_RS24265 Protein ID WP_043018956.1
Coordinates 4948990..4949232 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQ249_RS24245 4946119..4947021 + 903 WP_106107909.1 formate dehydrogenase O subunit beta -
EQ249_RS24250 4947018..4947653 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
EQ249_RS24255 4947650..4948579 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
EQ249_RS24260 4948616..4948990 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
EQ249_RS24265 4948990..4949232 - 243 WP_043018956.1 ribbon-helix-helix domain-containing protein Antitoxin
EQ249_RS24270 4949438..4950346 + 909 WP_106107908.1 alpha/beta hydrolase -
EQ249_RS24280 4950497..4951438 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
EQ249_RS24285 4951483..4951920 - 438 WP_003840439.1 D-tyrosyl-tRNA(Tyr) deacylase -
EQ249_RS24290 4951917..4952789 - 873 WP_003028669.1 virulence factor BrkB family protein -
EQ249_RS24295 4952783..4953382 - 600 WP_016151258.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T117535 WP_003028682.1 NZ_CP035276:c4948990-4948616 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T117535 NZ_CP035276:c4948990-4948616 [Citrobacter freundii]
ATGGTCAAAGGATCCGCGCTTTTTGATACCAACATCCTCATCGATCTGTTTAGTGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAATGCAATCAGCCTCATTACATGGATGGAGGTGATGGTGGGTGCAAAAAAATACCATCAGG
AAGCCCGTACTCGTGTTGCGCTAGGTGCTTTTAACGTGATCGGCGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGACAGGAATATGGGATGAAATTGCCGGATGCCATTATTTTGGCAACAGCACAGATTCATCGTCTTACGCTGGTTACGCG
AAATACTAAGGATTTTGCAGGCCTATCCGGTGTGGTCACACCGTATACATTATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT117535 WP_043018956.1 NZ_CP035276:c4949232-4948990 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT117535 NZ_CP035276:c4949232-4948990 [Citrobacter freundii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAAGTGATTAAACGTCTTGATGATCTCAAGGT
GCAGCGCAACCTGCCGCGAGCAGAACTTTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAGGATCAGGCTGAAACCA
CAATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTCCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References