Detailed information of TA system TA117534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 4797127..4797793
• Replicon: chromosome
• Accession: NZ_CP035276
• Organism: Citrobacter freundii strain R17

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A0D7LIM5
• Locus tag: EQ249_RS23550
• Protein ID: WP_044702260.1
• Coordinates: 4797434..4797793 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: EQ249_RS23545
• Protein ID: WP_106108075.1
• Coordinates: 4797127..4797444 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQ249_RS23515	4792306..4793103	+	798	WP_003841402.1	PTS mannose/fructose/sorbose transporter subunit IIC	-
EQ249_RS23520	4793115..4793939	+	825	WP_003031641.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
EQ249_RS23525	4794007..4795230	+	1224	WP_106108078.1	L-sorbose 1-phosphate reductase	-
EQ249_RS23530	4795232..4796044	+	813	WP_106108077.1	shikimate 5-dehydrogenase	-
EQ249_RS23535	4796094..4796450	-	357	WP_106108076.1	hypothetical protein	-
EQ249_RS26445	4796582..4796755	+	174	WP_032938222.1	hypothetical protein	-
EQ249_RS23540	4796826..4796987	+	162	WP_003841416.1	phage protein	-
EQ249_RS23545	4797127..4797444	-	318	WP_106108075.1	helix-turn-helix domain-containing protein	Antitoxin
EQ249_RS23550	4797434..4797793	-	360	WP_044702260.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EQ249_RS23555	4798007..4798696	+	690	WP_106108074.1	dipeptidase PepE	-
EQ249_RS23560	4798762..4800393	-	1632	WP_106108073.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-90)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13437.49 Da
• Isoelectric Point: 10.2555

>T117534 WP_044702260.1 NZ_CP035276:c4797793-4797434 [Citrobacter freundii]
MTKPLYWVGQARKDLIAMPEHVRDTFGFALWLAQQGKQHSQTKPLKGFGGAGVLEVVEDYHGNAWRAVYTIQLKNAVYVL
HVFQKKSVSGKATPKPEIDLIYQRLKAAQRHAQESGYVT

Nucleotide

• Length: 360 bp

>T117534 NZ_CP035276:c4797793-4797434 [Citrobacter freundii]
ATGACGAAACCACTTTACTGGGTTGGGCAGGCCAGAAAAGACCTGATTGCAATGCCAGAACACGTTCGGGATACGTTTGG
GTTTGCTCTTTGGCTTGCGCAGCAGGGTAAACAGCATTCACAGACTAAACCATTAAAAGGATTTGGTGGAGCGGGGGTTC
TGGAAGTCGTTGAGGATTATCACGGCAACGCCTGGCGGGCGGTTTACACCATCCAGTTGAAAAATGCGGTTTATGTTCTC
CATGTTTTTCAGAAGAAGTCCGTTTCCGGGAAAGCAACCCCGAAACCTGAAATTGACCTGATTTATCAGCGGCTCAAGGC
TGCACAGCGCCATGCGCAGGAGAGTGGATATGTCACATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11467.29 Da
• Isoelectric Point: 6.9791

>AT117534 WP_106108075.1 NZ_CP035276:c4797444-4797127 [Citrobacter freundii]
MSHDVETSSGNVYADLGAYDAEQMQLKAQLAMVIGEILQRKGLTQQQAAQLLGMTQPKLSNLLRGQFRGISEAKMLECLV
RLGRDVEIVVGEEGNMSGKLKVKFG

Nucleotide

• Length: 318 bp

>AT117534 NZ_CP035276:c4797444-4797127 [Citrobacter freundii]
ATGTCACATGATGTCGAAACCAGTAGCGGTAATGTTTACGCCGATTTAGGTGCGTATGACGCAGAACAGATGCAGTTGAA
AGCGCAGCTGGCAATGGTGATTGGTGAGATTTTGCAACGCAAAGGGTTAACGCAGCAGCAGGCCGCGCAGTTACTGGGAA
TGACACAGCCTAAACTTTCCAATCTGCTACGCGGCCAGTTTCGCGGTATCAGTGAAGCAAAGATGCTCGAATGCCTGGTG
AGACTGGGCCGTGATGTAGAGATTGTTGTCGGGGAAGAGGGCAATATGTCAGGAAAGCTGAAAGTGAAATTTGGCTGA

Structures

Toxin

• AlphaFold DB: A0A0D7LIM5