Detailed information of TA system TA117531

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3969709..3970415
• Replicon: chromosome
• Accession: NZ_CP035276
• Organism: Citrobacter freundii strain R17

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A7D6YAQ1
• Locus tag: EQ249_RS19595
• Protein ID: WP_071886833.1
• Coordinates: 3969709..3970077 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A7D6YLI3
• Locus tag: EQ249_RS19600
• Protein ID: WP_045341350.1
• Coordinates: 3970098..3970415 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQ249_RS19575	3964846..3966297	+	1452	WP_106107719.1	Na+/H+ antiporter NhaC	-
EQ249_RS19580	3966310..3967491	+	1182	WP_106107718.1	pyridoxal phosphate-dependent aminotransferase	-
EQ249_RS19585	3967657..3969135	-	1479	WP_106107717.1	hypothetical protein	-
EQ249_RS19595	3969709..3970077	-	369	WP_071886833.1	TA system toxin CbtA family protein	Toxin
EQ249_RS19600	3970098..3970415	-	318	WP_045341350.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EQ249_RS19605	3970433..3970654	-	222	WP_045341352.1	DUF987 domain-containing protein	-
EQ249_RS19610	3970663..3971145	-	483	WP_045341354.1	DNA repair protein RadC	-
EQ249_RS19615	3971154..3971603	-	450	WP_045341356.1	antirestriction protein	-
EQ249_RS19620	3971703..3971942	-	240	Protein_3762	DUF905 domain-containing protein	-
EQ249_RS19625	3972019..3972486	-	468	WP_045341358.1	hypothetical protein	-
EQ249_RS19630	3972510..3972950	-	441	WP_045341360.1	hypothetical protein	-
EQ249_RS19635	3972950..3973186	-	237	WP_045341362.1	hypothetical protein	-
EQ249_RS19640	3973227..3973928	-	702	Protein_3766	WYL domain-containing protein	-
EQ249_RS19645	3974145..3974966	-	822	WP_045341364.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3960572..3981817	21245

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13738.90 Da
• Isoelectric Point: 8.1347

>T117531 WP_071886833.1 NZ_CP035276:c3970077-3969709 [Citrobacter freundii]
MKTLPATISRAAKPCLSPVAVWQMLLTRLLEKHYGLTLNDTPFCDQTVIQEHIDAGITLANAVNFLVEKYELVRIDRTGF
TSQEQAPYLTVTDILHARRACGLMNSCSYREVSNIVLSRSRQ

Nucleotide

• Length: 369 bp

>T117531 NZ_CP035276:c3970077-3969709 [Citrobacter freundii]
ATGAAAACTCTACCTGCTACAATTTCGCGGGCGGCGAAGCCCTGTCTGTCTCCAGTGGCTGTCTGGCAAATGCTACTTAC
ACGCCTGCTGGAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTCTGTGATCAAACCGTTATACAGGAACATATCG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAAAAGTATGAGTTAGTACGTATCGATCGCACAGGATTT
ACCTCACAGGAACAAGCGCCATATTTGACCGTGACAGATATCCTTCATGCCAGGAGAGCATGCGGATTAATGAACAGTTG
TTCGTATCGGGAAGTAAGTAATATTGTTCTTAGCCGATCGCGGCAGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11783.42 Da
• Isoelectric Point: 6.6236

>AT117531 WP_045341350.1 NZ_CP035276:c3970415-3970098 [Citrobacter freundii]
MNNTTWGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSDAECLKLDVAFPHFISLMESMLTTGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYLYIVVYPTQS

Nucleotide

• Length: 318 bp

>AT117531 NZ_CP035276:c3970415-3970098 [Citrobacter freundii]
ATGAATAACACCACATGGGGCCTGCAGCGGGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGTAACCGGCT
GCACTATCTGGCTGACCGGGCAAGTATCACAGGTAAGTTTAGTGATGCCGAATGCCTGAAGTTGGATGTCGCATTCCCGC
ATTTTATCAGTCTGATGGAATCAATGCTGACTACTGGTGAAATGAACCCTCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGCTGCGGCTACTTATATATCGTTGTTTATCCCACTCAAAGCTAA

Structures

Toxin

• AlphaFold DB: A0A7D6YAQ1

Antitoxin

• AlphaFold DB: A0A7D6YLI3