Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3768199..3768819 Replicon chromosome
Accession NZ_CP035276
Organism Citrobacter freundii strain R17

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag EQ249_RS18660 Protein ID WP_002892050.1
Coordinates 3768601..3768819 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag EQ249_RS18655 Protein ID WP_003021733.1
Coordinates 3768199..3768573 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQ249_RS18645 3763345..3764538 + 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
EQ249_RS18650 3764561..3767710 + 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
EQ249_RS18655 3768199..3768573 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
EQ249_RS18660 3768601..3768819 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
EQ249_RS18665 3769001..3769552 + 552 WP_032936967.1 maltose O-acetyltransferase -
EQ249_RS18670 3769669..3770139 + 471 WP_003021724.1 YlaC family protein -
EQ249_RS18675 3770218..3770358 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
EQ249_RS18680 3770360..3770620 - 261 WP_003021719.1 type B 50S ribosomal protein L31 -
EQ249_RS26440 3770809..3770979 + 171 WP_164919152.1 hypothetical protein -
EQ249_RS18685 3771037..3772235 + 1199 WP_106108126.1 IS3 family transposase -
EQ249_RS18690 3772245..3773645 + 1401 Protein_3581 EAL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3771609..3772235 626


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T117530 WP_002892050.1 NZ_CP035276:3768601-3768819 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T117530 NZ_CP035276:3768601-3768819 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACTATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT117530 WP_003021733.1 NZ_CP035276:3768199-3768573 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT117530 NZ_CP035276:3768199-3768573 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References