Detailed information of TA system TA117519

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 820216..820971
• Replicon: chromosome
• Accession: NZ_CP035276
• Organism: Citrobacter freundii strain R17

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7L6ILI8
• Locus tag: EQ249_RS04085
• Protein ID: WP_047359063.1
• Coordinates: 820216..820593 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8I0KKX4
• Locus tag: EQ249_RS04090
• Protein ID: WP_073970862.1
• Coordinates: 820645..820971 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQ249_RS04060	815653..816690	-	1038	WP_106107605.1	glycosyl transferase family 2	-
EQ249_RS04065	816696..817811	-	1116	WP_106107606.1	FAD-binding oxidoreductase	-
EQ249_RS04070	818569..819417	-	849	WP_106107607.1	DUF4942 domain-containing protein	-
EQ249_RS04075	819496..819699	-	204	WP_047359061.1	DUF957 domain-containing protein	-
EQ249_RS04080	819728..820219	-	492	WP_047359062.1	hypothetical protein	-
EQ249_RS04085	820216..820593	-	378	WP_047359063.1	TA system toxin CbtA family protein	Toxin
EQ249_RS04090	820645..820971	-	327	WP_073970862.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EQ249_RS04095	821028..821249	-	222	WP_000691982.1	DUF987 domain-containing protein	-
EQ249_RS04100	821270..821749	-	480	WP_047359065.1	DNA repair protein RadC	-
EQ249_RS04105	821761..822222	-	462	WP_047359066.1	antirestriction protein	-
EQ249_RS04110	822319..822558	-	240	WP_047359067.1	DUF905 domain-containing protein	-
EQ249_RS04115	822636..823109	-	474	WP_052951289.1	hypothetical protein	-
EQ249_RS04120	823131..823826	-	696	WP_047359068.1	hypothetical protein	-
EQ249_RS04125	824009..824710	-	702	WP_047359069.1	WYL domain-containing protein	-
EQ249_RS04130	824926..825747	-	822	WP_032751326.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	812877..829487	16610

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (2-110)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14078.86 Da
• Isoelectric Point: 7.0606

>T117519 WP_047359063.1 NZ_CP035276:c820593-820216 [Citrobacter freundii]
VQTQPLSSTHETSPRPSPVDIWQHLLNHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
SADTQSPLIGSIDILRARKATGLMTRHGYRPVTDLITGKYKKEQQ

Nucleotide

• Length: 378 bp

>T117519 NZ_CP035276:c820593-820216 [Citrobacter freundii]
GTGCAAACCCAACCACTATCCTCCACGCATGAGACCTCACCACGTCCGTCCCCCGTGGATATCTGGCAGCACCTCCTGAA
CCATCTGCTGGATCGTCACTACGGTCTGACGCTAAACGACACGCCATTTGGCAACGATGGCGTGATCCAGGAGCATATCG
ACGCCGGTATCTCACTGTGCGACGCGGTGAACTTTATCGTGGAAAAGTACGACCTGGTCCGTACTGACAGACGCGGCTTT
AGCGCAGACACACAGTCACCGCTTATCGGCAGTATTGATATCCTCCGAGCCCGGAAGGCGACCGGGCTGATGACCCGTCA
TGGCTACAGACCTGTGACAGATCTCATTACCGGCAAATACAAAAAGGAACAACAATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11969.65 Da
• Isoelectric Point: 6.9554

>AT117519 WP_073970862.1 NZ_CP035276:c820971-820645 [Citrobacter freundii]
ITEPWWGLKRSITPCFGARLVQEGNRLHYLADRASIAGTFSDADLRHLDQAFPVQLKQLELMLVSGELNPRHQHCVTLYS
KGLTCEADSLGSHGYVYIAIYPTPAATA

Nucleotide

• Length: 327 bp

>AT117519 NZ_CP035276:c820971-820645 [Citrobacter freundii]
ATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGCAACCGGCT
GCACTATCTCGCCGACCGCGCGAGTATCGCCGGCACGTTCAGCGACGCGGATTTACGCCATCTTGACCAGGCGTTTCCGG
TACAGCTCAAACAGCTTGAGCTGATGCTGGTTTCCGGTGAACTCAATCCCCGCCATCAGCACTGCGTCACATTATACTCA
AAAGGGCTGACCTGCGAGGCCGACAGCCTCGGCTCGCATGGATACGTGTACATCGCGATTTACCCGACACCTGCAGCAAC
CGCGTAA

Structures

Toxin

• AlphaFold DB: A0A7L6ILI8