Detailed information of TA system TA117517

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 72337..73004
• Replicon: chromosome
• Accession: NZ_CP035276
• Organism: Citrobacter freundii strain R17

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A745GLG6
• Locus tag: EQ249_RS00350
• Protein ID: WP_024156847.1
• Coordinates: 72337..72657 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: EQ249_RS00355
• Protein ID: WP_023181597.1
• Coordinates: 72678..73004 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQ249_RS00325	69841..70215	-	375	WP_016151201.1	hypothetical protein	-
EQ249_RS00330	70621..70797	-	177	WP_044700470.1	hypothetical protein	-
EQ249_RS00335	70915..71205	-	291	WP_044700468.1	SymE family type I addiction module toxin	-
EQ249_RS00340	71342..71536	+	195	WP_044700466.1	toxin-antitoxin system HicB family antitoxin	-
EQ249_RS00345	71545..72111	-	567	WP_049015891.1	hypothetical protein	-
EQ249_RS00350	72337..72657	-	321	WP_024156847.1	TA system toxin CbtA family protein	Toxin
EQ249_RS00355	72678..73004	-	327	WP_023181597.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EQ249_RS00360	73017..73487	-	471	WP_015703840.1	DNA repair protein RadC	-
EQ249_RS00365	73556..74377	-	822	WP_106107940.1	DUF945 domain-containing protein	-
EQ249_RS00370	74449..74898	-	450	WP_023181599.1	hypothetical protein	-
EQ249_RS00380	75142..75945	-	804	WP_063940259.1	hypothetical protein	-
EQ249_RS00385	75965..76321	-	357	Protein_75	hypothetical protein	-
EQ249_RS00390	76803..77945	-	1143	WP_023181602.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	misL	64876..86587	21711

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-98)

Antitoxin

• Antitoxin CbeA (7-107)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12066.80 Da
• Isoelectric Point: 5.0482

>T117517 WP_024156847.1 NZ_CP035276:c72657-72337 [Citrobacter freundii]
MNTQPATTPQAAKPCLSPLAVWQMLLTSLLDQHYGLTLNDTPFSDETVIQKHIEAGITLADAVNFLVERYELVRIDQKGF
SVQDQEPWLTSMDVHRARFKLNVERL

Nucleotide

• Length: 321 bp

>T117517 NZ_CP035276:c72657-72337 [Citrobacter freundii]
ATGAATACTCAACCTGCAACAACTCCGCAGGCGGCGAAGCCATGTCTGTCGCCCCTGGCTGTCTGGCAAATGTTACTGAC
AAGCCTGCTAGACCAGCACTATGGTCTCACGCTAAACGACACGCCATTCAGTGATGAAACTGTTATTCAGAAACACATTG
AGGCAGGTATTACCTTGGCCGATGCCGTAAACTTTCTGGTTGAACGCTATGAGCTAGTTCGTATTGACCAAAAAGGCTTC
TCTGTGCAAGATCAAGAACCTTGGTTAACTTCTATGGATGTCCACCGAGCACGATTTAAACTCAACGTGGAACGTTTATA
A

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12353.05 Da
• Isoelectric Point: 5.8083

>AT117517 WP_023181597.1 NZ_CP035276:c73004-72678 [Citrobacter freundii]
MSLISAHEWGLQREIVPRFGARLVQEGNRLHYLADRANLMGIFSDTECFKLDDAFPHFISQMESMLTTGELIPCHHHCVT
LYHNDFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 327 bp

>AT117517 NZ_CP035276:c73004-72678 [Citrobacter freundii]
ATGTCATTAATATCAGCCCATGAATGGGGACTACAAAGAGAAATTGTTCCACGGTTTGGTGCCAGATTAGTTCAGGAAGG
CAACCGGCTGCATTATCTGGCTGACAGGGCTAATTTGATGGGCATTTTCAGCGATACGGAATGCTTCAAATTGGATGACG
CATTTCCACACTTTATCAGTCAGATGGAATCGATGCTGACTACTGGTGAACTGATCCCCTGTCACCATCACTGTGTCACT
CTCTACCACAACGATTTTACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTGTACATCGCGGTTTATCCCACTCA
ACGCTAA

Structures

Toxin

• AlphaFold DB: A0A745GLG6