Detailed information of TA system TA117516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1617611..1618279
• Replicon: chromosome
• Accession: NZ_CP035265
• Organism: Streptococcus pneumoniae strain TVO_1901920

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: C1C927
• Locus tag: EQH15_RS08225
• Protein ID: WP_001132282.1
• Coordinates: 1618100..1618279 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EQH15_RS08220
• Protein ID: WP_000961808.1
• Coordinates: 1617611..1618063 (-)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQH15_RS08195 (EQH15_08610)	1613501..1614244	-	744	WP_001188213.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
EQH15_RS08200 (EQH15_08615)	1614246..1615199	-	954	WP_000970781.1	50S ribosomal protein L11 methyltransferase	-
EQH15_RS08205 (EQH15_08620)	1615334..1615762	-	429	WP_000418168.1	NUDIX hydrolase	-
EQH15_RS08210 (EQH15_08625)	1615743..1616831	-	1089	WP_000719722.1	site-2 protease family protein	-
EQH15_RS08215 (EQH15_08630)	1616850..1617320	-	471	WP_000257097.1	DUF3013 family protein	-
EQH15_RS08220 (EQH15_08635)	1617611..1618063	-	453	WP_000961808.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EQH15_RS08225 (EQH15_08640)	1618100..1618279	-	180	WP_001132282.1	type II toxin-antitoxin system HicA family toxin	Toxin
EQH15_RS08230 (EQH15_08645)	1618416..1618595	-	180	WP_001048906.1	hypothetical protein	-
EQH15_RS08235 (EQH15_08655)	1618799..1618999	-	201	WP_000818081.1	hypothetical protein	-
EQH15_RS08240 (EQH15_08665)	1619279..1620550	+	1272	WP_001113212.1	replication-associated recombination protein A	-
EQH15_RS08250 (EQH15_08675)	1621095..1622414	-	1320	WP_000608889.1	glycoside hydrolase family 32 protein	-
EQH15_RS08255 (EQH15_08680)	1622424..1623269	-	846	WP_000819520.1	carbohydrate ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6713.95 Da
• Isoelectric Point: 11.0174

>T117516 WP_001132282.1 NZ_CP035265:c1618279-1618100 [Streptococcus pneumoniae]
MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERLITIPHGELNKYTERGIRKQAGL

Nucleotide

• Length: 180 bp

>T117516 NZ_CP035265:c1618279-1618100 [Streptococcus pneumoniae]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCA
TATTAAAATGGAGAAGCAAGGGGAAAGACTTATCACAATCCCTCATGGTGAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16685.54 Da
• Isoelectric Point: 3.7220

>AT117516 WP_000961808.1 NZ_CP035265:c1618063-1617611 [Streptococcus pneumoniae]
MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAMGSEWLGITVADLIESDGELPQPSDINSLSLIDNDPF
KDDEDFVSTYDLDKSFISMVSVDVLEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA

Nucleotide

• Length: 453 bp

>AT117516 NZ_CP035265:c1618063-1617611 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATTAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACTATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Structures

Toxin

• AlphaFold DB: A0A0E7SUI3