Detailed information of TA system TA117508

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 1005832..1007069
• Replicon: chromosome
• Accession: NZ_CP035264
• Organism: Streptococcus pneumoniae strain TVO_1901921

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: Q97QZ1
• Locus tag: EQH16_RS05250
• Protein ID: WP_000405360.1
• Coordinates: 1006308..1007069 (+)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: B6ZCC4
• Locus tag: EQH16_RS05245
• Protein ID: WP_000579612.1
• Coordinates: 1005832..1006308 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQH16_RS05225 (EQH16_05485)	1000905..1001759	+	855	Protein_1040	ADP-ribosylglycohydrolase family protein	-
EQH16_RS05230 (EQH16_05490)	1001871..1002755	+	885	WP_000710115.1	diacylglycerol kinase family lipid kinase	-
EQH16_RS05235 (EQH16_05495)	1002918..1004657	+	1740	WP_000423465.1	glycoside hydrolase family 13 protein	-
EQH16_RS05240 (EQH16_05500)	1004932..1005387	+	456	WP_000500478.1	hypothetical protein	-
EQH16_RS05245 (EQH16_05505)	1005832..1006308	+	477	WP_000579612.1	type II toxin-antitoxin system antitoxin PezA	Antitoxin
EQH16_RS05250 (EQH16_05510)	1006308..1007069	+	762	WP_000405360.1	type II toxin-antitoxin system toxin PezT	Toxin
EQH16_RS05255 (EQH16_05515)	1007097..1008261	+	1165	Protein_1046	hypothetical protein	-
EQH16_RS05260 (EQH16_05520)	1008739..1009014	+	276	WP_000500547.1	hypothetical protein	-
EQH16_RS05265 (EQH16_05525)	1009080..1009439	+	360	WP_000003745.1	SAG1252 family conjugative relaxosome accessory protein	-
EQH16_RS05270 (EQH16_05530)	1009449..1009814	+	366	WP_000263049.1	plasmid mobilization relaxosome protein MobC	-
EQH16_RS05275 (EQH16_05535)	1009801..1011666	+	1866	WP_000261795.1	SAG1250 family conjugative relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	997004..1011666	14662

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 29129.20 Da
• Isoelectric Point: 7.5087

>T117508 WP_000405360.1 NZ_CP035264:1006308-1007069 [Streptococcus pneumoniae]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRKLEELAIFERIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
QVGEKRLNELLEK

Nucleotide

• Length: 762 bp

>T117508 NZ_CP035264:1006308-1007069 [Streptococcus pneumoniae]
ATGGAAATCCAAGATTATACTGATAGTGAATTCAAACATGCTTTAGCAAGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAGCAACCTATAGCGATTTTGCTTGGAGGGCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGTGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGACAGTGTAGAATATACCAAAGATTTTGCAGGAAAAATGGTAGAGTCTTTAGTAACAAAATTGAGTAG
TTTGAGATACAATCTTTTGATCGAGGGAACTTTACGAACAGTTGATGTTCCAAAGAAAACAGCACAACTCTTGAAAAATA
AGGGATATGAAGTACAATTGGCCTTAATTGCGACAAAGCCTGAATTGTCGTATCTAAGTACTCTTATCCGTTATGAAGAA
CTGTACATTATCAATCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
CACACGAAAATTGGAAGAACTAGCTATCTTTGAAAGAATTCAAATTTACCAACGAGATAGAAGTTGTGTATATGATTCAA
AAGAAAATACAACTTCAGCAGCAGATGTTCTTCAAGAGTTACTCTTTGGGGAGTGGAGTCAGGTAGAGAAGGAGATGTTG
CAGGTGGGGGAAAAGAGACTTAATGAATTACTTGAAAAATAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18245.68 Da
• Isoelectric Point: 4.4188

>AT117508 WP_000579612.1 NZ_CP035264:1005832-1006308 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT117508 NZ_CP035264:1005832-1006308 [Streptococcus pneumoniae]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACACAACCCGAATTTGCACGAATTATAGGAATTTC
TCGAAATAGCTTGAGTCGTTATGAAAATGGAACTAGTTCAGTCTCTACGGAACTAATAGATATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTGAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTATTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGACTTGATTCATACGAATATCTATTTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGTGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAGCGGATGGTAGCTTAA

Structures

Toxin

• PDB: 2P5T

Antitoxin

• AlphaFold DB: B6ZCC4