Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1540706..1541374 Replicon chromosome
Accession NZ_CP035260
Organism Streptococcus pneumoniae strain TVO_1901925

Toxin (Protein)


Gene name hicA Uniprot ID A0A0B7M7X4
Locus tag EQH20_RS07680 Protein ID WP_001132284.1
Coordinates 1541195..1541374 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID G0IC64
Locus tag EQH20_RS07675 Protein ID WP_000961810.1
Coordinates 1540706..1541158 (-) Length 151 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQH20_RS07650 (EQH20_08090) 1536598..1537341 - 744 WP_001188210.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
EQH20_RS07655 (EQH20_08095) 1537343..1538293 - 951 WP_000451152.1 50S ribosomal protein L11 methyltransferase -
EQH20_RS07660 (EQH20_08100) 1538429..1538857 - 429 WP_000418174.1 NUDIX hydrolase -
EQH20_RS07665 (EQH20_08105) 1538838..1539926 - 1089 WP_000719713.1 site-2 protease family protein -
EQH20_RS07670 (EQH20_08110) 1539945..1540415 - 471 WP_000257086.1 DUF3013 family protein -
EQH20_RS07675 (EQH20_08115) 1540706..1541158 - 453 WP_000961810.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EQH20_RS07680 (EQH20_08120) 1541195..1541374 - 180 WP_001132284.1 type II toxin-antitoxin system HicA family toxin Toxin
EQH20_RS07685 (EQH20_08125) 1541511..1541690 - 180 WP_001048906.1 hypothetical protein -
EQH20_RS07690 (EQH20_08135) 1541855..1542076 - 222 WP_000425689.1 hypothetical protein -
EQH20_RS07695 (EQH20_08140) 1542368..1543639 + 1272 WP_050240732.1 replication-associated recombination protein A -
EQH20_RS07705 (EQH20_08150) 1544184..1545503 - 1320 WP_000502556.1 glycoside hydrolase family 32 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(17-114)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6713.95 Da        Isoelectric Point: 11.0174

>T117495 WP_001132284.1 NZ_CP035260:c1541374-1541195 [Streptococcus pneumoniae]
MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGELNKYTERGIRKQAGL

Download         Length: 180 bp

>T117495 NZ_CP035260:c1541374-1541195 [Streptococcus pneumoniae]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCA
TATTAAAATGGAAAAGCAAGGGGAAAGACCTATCACAATCCTTCATGGTGAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin


Download         Length: 151 a.a.        Molecular weight: 16659.46 Da        Isoelectric Point: 3.7220

>AT117495 WP_000961810.1 NZ_CP035260:c1541158-1540706 [Streptococcus pneumoniae]
MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAMGSEWLGITVADLIESDGELPQPSDINSLSLIDNDPF
KDDEDFVSTYDLDKSFISMVSVDVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA

Download         Length: 453 bp

>AT117495 NZ_CP035260:c1541158-1540706 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACCATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGGCTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B7M7X4

Antitoxin

Source ID Structure
PDB 5YRZ
AlphaFold DB G0IC64

References