Detailed information of TA system TA117492

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1628490..1629158
• Replicon: chromosome
• Accession: NZ_CP035259
• Organism: Streptococcus pneumoniae strain TVO_1901926

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: I0T4C6
• Locus tag: EQH21_RS08140
• Protein ID: WP_001132287.1
• Coordinates: 1628979..1629158 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: G0IC64
• Locus tag: EQH21_RS08135
• Protein ID: WP_000961810.1
• Coordinates: 1628490..1628942 (-)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQH21_RS08110 (EQH21_08505)	1624380..1625123	-	744	WP_001188205.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
EQH21_RS08115 (EQH21_08510)	1625125..1626078	-	954	WP_000970773.1	50S ribosomal protein L11 methyltransferase	-
EQH21_RS08120 (EQH21_08515)	1626213..1626641	-	429	WP_000401987.1	NUDIX hydrolase	-
EQH21_RS08125 (EQH21_08520)	1626643..1627710	-	1068	WP_000719703.1	site-2 protease family protein	-
EQH21_RS08130 (EQH21_08525)	1627729..1628199	-	471	WP_000257083.1	DUF3013 family protein	-
EQH21_RS08135 (EQH21_08530)	1628490..1628942	-	453	WP_000961810.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EQH21_RS08140 (EQH21_08535)	1628979..1629158	-	180	WP_001132287.1	type II toxin-antitoxin system HicA family toxin	Toxin
EQH21_RS08145 (EQH21_08540)	1629295..1629474	-	180	WP_001810816.1	hypothetical protein	-
EQH21_RS08150 (EQH21_08550)	1629639..1629878	-	240	WP_000818079.1	hypothetical protein	-
EQH21_RS08155 (EQH21_08560)	1630148..1631419	+	1272	WP_001113205.1	replication-associated recombination protein A	-
EQH21_RS08165 (EQH21_08570)	1631841..1632968	-	1128	WP_000323403.1	tyrosine-type recombinase/integrase	-
EQH21_RS08170 (EQH21_08575)	1632970..1633152	-	183	WP_000655518.1	DUF3173 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (17-114)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6697.91 Da
• Isoelectric Point: 11.0174

>T117492 WP_001132287.1 NZ_CP035259:c1629158-1628979 [Streptococcus pneumoniae]
MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITIPHGELNKYTERGIRKQAGL

Nucleotide

• Length: 180 bp

>T117492 NZ_CP035259:c1629158-1628979 [Streptococcus pneumoniae]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGTTCCCA
TATTAAAATGGAGAAGCAAGGGGAAAGACCTATCACAATCCCTCATGGTGAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16659.46 Da
• Isoelectric Point: 3.7220

>AT117492 WP_000961810.1 NZ_CP035259:c1628942-1628490 [Streptococcus pneumoniae]
MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAMGSEWLGITVADLIESDGELPQPSDINSLSLIDNDPF
KDDEDFVSTYDLDKSFISMVSVDVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA

Nucleotide

• Length: 453 bp

>AT117492 NZ_CP035259:c1628942-1628490 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCCACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACCATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGATGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Structures

Toxin

• AlphaFold DB: I0T4C6

Antitoxin

• PDB: 5YRZ
• AlphaFold DB: G0IC64