Detailed information of TA system TA117489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1631314..1631982
• Replicon: chromosome
• Accession: NZ_CP035258
• Organism: Streptococcus pneumoniae strain TVO_1901927

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: I0T4C6
• Locus tag: EQH22_RS08125
• Protein ID: WP_001132287.1
• Coordinates: 1631803..1631982 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: G0IC64
• Locus tag: EQH22_RS08120
• Protein ID: WP_000961810.1
• Coordinates: 1631314..1631766 (-)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQH22_RS08095 (EQH22_08510)	1627204..1627947	-	744	WP_001188205.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
EQH22_RS08100 (EQH22_08515)	1627949..1628902	-	954	WP_000970773.1	50S ribosomal protein L11 methyltransferase	-
EQH22_RS08105 (EQH22_08520)	1629037..1629465	-	429	WP_000401987.1	NUDIX hydrolase	-
EQH22_RS08110 (EQH22_08525)	1629467..1630534	-	1068	WP_000719703.1	site-2 protease family protein	-
EQH22_RS08115 (EQH22_08530)	1630553..1631023	-	471	WP_000257083.1	DUF3013 family protein	-
EQH22_RS08120 (EQH22_08535)	1631314..1631766	-	453	WP_000961810.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EQH22_RS08125 (EQH22_08540)	1631803..1631982	-	180	WP_001132287.1	type II toxin-antitoxin system HicA family toxin	Toxin
EQH22_RS08130 (EQH22_08545)	1632119..1632298	-	180	WP_001810816.1	hypothetical protein	-
EQH22_RS08135 (EQH22_08555)	1632463..1632702	-	240	WP_000818079.1	hypothetical protein	-
EQH22_RS08140 (EQH22_08565)	1632972..1634243	+	1272	WP_001113205.1	replication-associated recombination protein A	-
EQH22_RS08150 (EQH22_08575)	1634791..1636110	-	1320	WP_000502555.1	glycoside hydrolase family 32 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (17-114)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6697.91 Da
• Isoelectric Point: 11.0174

>T117489 WP_001132287.1 NZ_CP035258:c1631982-1631803 [Streptococcus pneumoniae]
MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITIPHGELNKYTERGIRKQAGL

Nucleotide

• Length: 180 bp

>T117489 NZ_CP035258:c1631982-1631803 [Streptococcus pneumoniae]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGTTCCCA
TATTAAAATGGAGAAGCAAGGGGAAAGACCTATCACAATCCCTCATGGTGAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16659.46 Da
• Isoelectric Point: 3.7220

>AT117489 WP_000961810.1 NZ_CP035258:c1631766-1631314 [Streptococcus pneumoniae]
MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAMGSEWLGITVADLIESDGELPQPSDINSLSLIDNDPF
KDDEDFVSTYDLDKSFISMVSVDVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA

Nucleotide

• Length: 453 bp

>AT117489 NZ_CP035258:c1631766-1631314 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCCACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACCATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGATGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Structures

Toxin

• AlphaFold DB: I0T4C6

Antitoxin

• AlphaFold DB: G0IC64