Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1653429..1654097 Replicon chromosome
Accession NZ_CP035254
Organism Streptococcus pneumoniae strain TVO_1901932

Toxin (Protein)


Gene name hicA Uniprot ID C1C927
Locus tag EQH26_RS08290 Protein ID WP_001132282.1
Coordinates 1653918..1654097 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID G0IC64
Locus tag EQH26_RS08285 Protein ID WP_000961810.1
Coordinates 1653429..1653881 (-) Length 151 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQH26_RS08260 (EQH26_08730) 1649319..1650062 - 744 WP_001188211.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
EQH26_RS08265 (EQH26_08735) 1650064..1651017 - 954 WP_000970781.1 50S ribosomal protein L11 methyltransferase -
EQH26_RS08270 (EQH26_08740) 1651152..1651580 - 429 WP_000401987.1 NUDIX hydrolase -
EQH26_RS08275 (EQH26_08745) 1651582..1652649 - 1068 WP_000719703.1 site-2 protease family protein -
EQH26_RS08280 (EQH26_08750) 1652668..1653138 - 471 WP_000257100.1 DUF3013 family protein -
EQH26_RS08285 (EQH26_08755) 1653429..1653881 - 453 WP_000961810.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EQH26_RS08290 (EQH26_08760) 1653918..1654097 - 180 WP_001132282.1 type II toxin-antitoxin system HicA family toxin Toxin
EQH26_RS08295 (EQH26_08765) 1654234..1654413 - 180 WP_001048906.1 hypothetical protein -
EQH26_RS08300 (EQH26_08775) 1654617..1654817 - 201 WP_000818077.1 hypothetical protein -
EQH26_RS08305 (EQH26_08785) 1655087..1656358 + 1272 WP_001113204.1 replication-associated recombination protein A -
EQH26_RS08315 (EQH26_08795) 1656903..1658222 - 1320 WP_000608887.1 glycoside hydrolase family 32 protein -
EQH26_RS08320 (EQH26_08800) 1658232..1659077 - 846 WP_000819521.1 carbohydrate ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(17-114)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6713.95 Da        Isoelectric Point: 11.0174

>T117480 WP_001132282.1 NZ_CP035254:c1654097-1653918 [Streptococcus pneumoniae]
MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERLITIPHGELNKYTERGIRKQAGL

Download         Length: 180 bp

>T117480 NZ_CP035254:c1654097-1653918 [Streptococcus pneumoniae]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCA
TATTAAAATGGAGAAGCAAGGGGAAAGACTTATCACAATCCCTCATGGTGAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin


Download         Length: 151 a.a.        Molecular weight: 16659.46 Da        Isoelectric Point: 3.7220

>AT117480 WP_000961810.1 NZ_CP035254:c1653881-1653429 [Streptococcus pneumoniae]
MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAMGSEWLGITVADLIESDGELPQPSDINSLSLIDNDPF
KDDEDFVSTYDLDKSFISMVSVDVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA

Download         Length: 453 bp

>AT117480 NZ_CP035254:c1653881-1653429 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACTATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E7SUI3

Antitoxin

Source ID Structure
PDB 5YRZ
AlphaFold DB G0IC64

References