Detailed information of TA system TA117477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 998294..999531
• Replicon: chromosome
• Accession: NZ_CP035254
• Organism: Streptococcus pneumoniae strain TVO_1901932

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: A0A098ZIW8
• Locus tag: EQH26_RS05085
• Protein ID: WP_000405362.1
• Coordinates: 998770..999531 (+)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: EQH26_RS05080
• Protein ID: WP_000579613.1
• Coordinates: 998294..998770 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQH26_RS05060 (EQH26_05385)	993368..994222	+	855	WP_000900020.1	ADP-ribosylglycohydrolase family protein	-
EQH26_RS05065 (EQH26_05390)	994334..995218	+	885	WP_000710115.1	diacylglycerol kinase family lipid kinase	-
EQH26_RS05070 (EQH26_05395)	995381..997119	+	1739	Protein_1008	glycoside hydrolase family 13 protein	-
EQH26_RS05075 (EQH26_05400)	997394..997849	+	456	WP_000500474.1	hypothetical protein	-
EQH26_RS05080 (EQH26_05405)	998294..998770	+	477	WP_000579613.1	type II toxin-antitoxin system antitoxin PezA	Antitoxin
EQH26_RS05085 (EQH26_05410)	998770..999531	+	762	WP_000405362.1	type II toxin-antitoxin system toxin PezT	Toxin
EQH26_RS05090	999559..999849	+	291	WP_001213954.1	hypothetical protein	-
EQH26_RS05095	999843..1001102	+	1260	WP_001816651.1	AAA family ATPase	-
EQH26_RS05100	1001299..1002042	+	744	WP_001154376.1	hypothetical protein	-
EQH26_RS05105 (EQH26_05420)	1002863..1003222	+	360	WP_000003748.1	SAG1252 family conjugative relaxosome accessory protein	-
EQH26_RS05110 (EQH26_05425)	1003232..1003597	+	366	WP_000263055.1	plasmid mobilization relaxosome protein MobC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	979561..1096600	117039

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 29129.16 Da
• Isoelectric Point: 7.1308

>T117477 WP_000405362.1 NZ_CP035254:998770-999531 [Streptococcus pneumoniae]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
QVGEKRLNELLEK

Nucleotide

• Length: 762 bp

>T117477 NZ_CP035254:998770-999531 [Streptococcus pneumoniae]
ATGGAAATCCAAGATTATACTGATAGTGAATTCAAACATGCTTTAGCAAGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAACAACCTATAGCGATTTTGCTTGGAGGTCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGTGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGACAGTGTAGAATATACCAAAGATTTTGCAGGAAAAATGGTAGAGTCTTTAGTAACAAAATTGAGTAG
TTTGAGATACAATCTTTTGATAGAGGGAACTTTACGAACAGTTGATGTTCCAAAGAAAACAGCACAACTCTTGAAAAATA
AGGGATATGAAGTACAATTGGCCTTAATTGCGACAAAGCCTGAATTGTCGTATCTAAGTACTCTTATCCGTTATGAAGAA
CTGTACATTATCAATCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
TACACGACAATTGGAAGAACTGGCTATCTTTGAAAGAATTCAAATTTACCAACGAGATAGAAGTTGTGTATATGATTCAA
AAGAAAATACAACTTCAGCAGCAGATGTTCTTCAAGAGTTACTGTTTGGGGAGTGGAGTCAGGTAGAGAAGGAGATGTTG
CAGGTGGGGGAAAAGAGACTTAATGAATTACTTGAAAAATAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18249.67 Da
• Isoelectric Point: 4.3462

>AT117477 WP_000579613.1 NZ_CP035254:998294-998770 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT117477 NZ_CP035254:998294-998770 [Streptococcus pneumoniae]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACACAACCTGAATTTGCACGAATTATAGGAATTTC
TCGAAATAGCTTGAGTCGTTATGAAAATGGAACTAGTTCAGTCTCTACGGAATTAATTGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTGAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTATTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGACTTGATTCAGACAAATATCTATTTAGTAGAAAATTTTGATG
AAATAGAGAGATATAGCGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAGCGGATGGTAGCTTAA

Structures

Toxin

• AlphaFold DB: A0A098ZIW8