Detailed information of TA system TA117476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1670159..1670827
• Replicon: chromosome
• Accession: NZ_CP035252
• Organism: Streptococcus pneumoniae strain TVO_1901934

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: EQH28_RS08355
• Protein ID: WP_025169096.1
• Coordinates: 1670648..1670827 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: G0IC64
• Locus tag: EQH28_RS08350
• Protein ID: WP_000961810.1
• Coordinates: 1670159..1670611 (-)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQH28_RS08325 (EQH28_08830)	1666049..1666792	-	744	WP_001188207.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
EQH28_RS08330 (EQH28_08835)	1666794..1667744	-	951	WP_000451134.1	50S ribosomal protein L11 methyltransferase	-
EQH28_RS08335 (EQH28_08840)	1667882..1668310	-	429	WP_000401988.1	NUDIX hydrolase	-
EQH28_RS08340 (EQH28_08845)	1668312..1669379	-	1068	WP_000719721.1	site-2 protease family protein	-
EQH28_RS08345 (EQH28_08850)	1669398..1669868	-	471	WP_000257084.1	DUF3013 family protein	-
EQH28_RS08350 (EQH28_08855)	1670159..1670611	-	453	WP_000961810.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EQH28_RS08355 (EQH28_08860)	1670648..1670827	-	180	WP_025169096.1	type II toxin-antitoxin system HicA family toxin	Toxin
EQH28_RS08360 (EQH28_08865)	1670964..1671143	-	180	WP_001809903.1	hypothetical protein	-
EQH28_RS08365 (EQH28_08875)	1671308..1671546	-	239	Protein_1667	hypothetical protein	-
EQH28_RS08370 (EQH28_08885)	1671816..1673087	+	1272	WP_001113208.1	replication-associated recombination protein A	-
EQH28_RS08380 (EQH28_08895)	1673633..1674952	-	1320	WP_000502548.1	glycoside hydrolase family 32 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (17-114)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6578.75 Da
• Isoelectric Point: 10.8214

>T117476 WP_025169096.1 NZ_CP035252:c1670827-1670648 [Streptococcus pneumoniae]
IPMTQKEMVKLLTAHGWIKTRGGKGSHIKIEKQGERPITILHGELNKYTERGIGKQAGL

Nucleotide

• Length: 180 bp

>T117476 NZ_CP035252:c1670827-1670648 [Streptococcus pneumoniae]
ATACCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCA
TATTAAAATCGAGAAGCAAGGGGAAAGACCTATCACAATCCTTCATGGTGAGCTGAATAAGTACACTGAAAGAGGGATAG
GAAAGCAAGCTGGGTTGTAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16659.46 Da
• Isoelectric Point: 3.7220

>AT117476 WP_000961810.1 NZ_CP035252:c1670611-1670159 [Streptococcus pneumoniae]
MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAMGSEWLGITVADLIESDGELPQPSDINSLSLIDNDPF
KDDEDFVSTYDLDKSFISMVSVDVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA

Nucleotide

• Length: 453 bp

>AT117476 NZ_CP035252:c1670611-1670159 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACCATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Structures

Antitoxin

• AlphaFold DB: G0IC64