Detailed information of TA system TA117474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 956987..958224
• Replicon: chromosome
• Accession: NZ_CP035252
• Organism: Streptococcus pneumoniae strain TVO_1901934

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: EQH28_RS04830
• Protein ID: WP_000405365.1
• Coordinates: 957463..958224 (+)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: EQH28_RS04825
• Protein ID: WP_000579599.1
• Coordinates: 956987..957463 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQH28_RS04805 (EQH28_05135)	952050..952904	+	855	WP_000900017.1	ADP-ribosylglycohydrolase family protein	-
EQH28_RS04810 (EQH28_05140)	953016..953900	+	885	WP_000710115.1	diacylglycerol kinase family lipid kinase	-
EQH28_RS04815 (EQH28_05145)	954063..955802	+	1740	WP_000423466.1	glycoside hydrolase family 13 protein	-
EQH28_RS04820 (EQH28_05150)	956077..956397	+	321	WP_061751326.1	hypothetical protein	-
EQH28_RS04825 (EQH28_05155)	956987..957463	+	477	WP_000579599.1	type II toxin-antitoxin system antitoxin PezA	Antitoxin
EQH28_RS04830 (EQH28_05160)	957463..958224	+	762	WP_000405365.1	type II toxin-antitoxin system toxin PezT	Toxin
EQH28_RS04835 (EQH28_05165)	958252..959028	+	777	WP_001821017.1	hypothetical protein	-
EQH28_RS04840	959031..959408	+	378	WP_000530000.1	hypothetical protein	-
EQH28_RS04845 (EQH28_05175)	960227..960586	+	360	WP_000003745.1	SAG1252 family conjugative relaxosome accessory protein	-
EQH28_RS04850 (EQH28_05180)	960596..960961	+	366	WP_000263051.1	plasmid mobilization relaxosome protein MobC	-
EQH28_RS04855 (EQH28_05185)	960948..962813	+	1866	WP_000261794.1	SAG1250 family conjugative relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	945564..958224	12660

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 29060.05 Da
• Isoelectric Point: 6.8511

>T117474 WP_000405365.1 NZ_CP035252:957463-958224 [Streptococcus pneumoniae]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFESIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
QVGEKRLNELLEK

Nucleotide

• Length: 762 bp

>T117474 NZ_CP035252:957463-958224 [Streptococcus pneumoniae]
ATGGAAATCCAAGATTATACTGATAGTGAATTCAAACATGCTTTAGCAAGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAGCAACCTATAGCGATTTTGCTTGGAGGGCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGTGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGACAGTGTAGAATATACCAAAGATTTTGCAGGAAAAATGGTAGAGTCTTTAGTAACAAAATTGAGTAG
TTTGAGATACAATCTTTTGATCGAGGGAACTTTACGAACAGTTGATGTTCCAAAGAAAACAGCACAACTCTTGAAAAATA
AGGGATATGAAGTACAATTGGCCTTAATTGCGACAAAGCCTGAATTGTCGTATCTAAGTACTCTTATCCGTTATGAAGAA
CTGTACATTATCAATCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
TACACGACAATTGGAAGAACTGGCTATCTTTGAAAGCATTCAAATTTACCAACGAGATAGAAGTTGTGTATATGATTCAA
AAGAAAATACAACTTCAGCAGCAGATGTTCTTCAAGAGTTACTCTTTGGGGAGTGGAGTCAGGTAGAGAAGGAGATGTTG
CAGGTGGGGGAAAAGAGACTTAATGAATTACTTGAAAAATAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18251.60 Da
• Isoelectric Point: 4.3571

>AT117474 WP_000579599.1 NZ_CP035252:956987-957463 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQDDFSRIVGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT117474 NZ_CP035252:956987-957463 [Streptococcus pneumoniae]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACACAAGACGACTTTTCCCGAATTGTAGGAATTTC
TCGAAATAGCTTGAGCCGGTATGAAAATGGAACTAGTTCAGTCTCTACGGAACTAATAGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTGAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTATTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGACTTGATTCATACGAATATCTATTTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGTGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAGCGGATGGTAGCTTAA