Detailed information of TA system TA117460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1583119..1583787
• Replicon: chromosome
• Accession: NZ_CP035245
• Organism: Streptococcus pneumoniae strain TVO_1901943

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B1I7Q0
• Locus tag: EQH35_RS08110
• Protein ID: WP_001132285.1
• Coordinates: 1583608..1583787 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A4J1XUY0
• Locus tag: EQH35_RS08105
• Protein ID: WP_000961812.1
• Coordinates: 1583119..1583571 (-)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQH35_RS08080 (EQH35_08520)	1579009..1579752	-	744	WP_001188204.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
EQH35_RS08085 (EQH35_08525)	1579754..1580704	-	951	WP_000451160.1	50S ribosomal protein L11 methyltransferase	-
EQH35_RS08090 (EQH35_08530)	1580842..1581270	-	429	WP_000418157.1	NUDIX hydrolase	-
EQH35_RS08095 (EQH35_08535)	1581251..1582339	-	1089	WP_000719712.1	site-2 protease family protein	-
EQH35_RS08100 (EQH35_08540)	1582358..1582828	-	471	WP_000257088.1	DUF3013 family protein	-
EQH35_RS08105 (EQH35_08545)	1583119..1583571	-	453	WP_000961812.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EQH35_RS08110 (EQH35_08550)	1583608..1583787	-	180	WP_001132285.1	type II toxin-antitoxin system HicA family toxin	Toxin
EQH35_RS08115 (EQH35_08555)	1583924..1584172	-	249	WP_000797237.1	hypothetical protein	-
EQH35_RS08120 (EQH35_08560)	1584162..1584401	-	240	WP_000818079.1	hypothetical protein	-
EQH35_RS08125 (EQH35_08570)	1584671..1585942	+	1272	WP_001113213.1	replication-associated recombination protein A	-
EQH35_RS08135 (EQH35_08580)	1586362..1587489	-	1128	WP_000323403.1	tyrosine-type recombinase/integrase	-
EQH35_RS08140 (EQH35_08585)	1587491..1587673	-	183	WP_000655518.1	DUF3173 domain-containing protein	-
EQH35_RS08145 (EQH35_08590)	1587689..1588732	-	1044	WP_001031659.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (17-114)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6713.01 Da
• Isoelectric Point: 11.2964

>T117460 WP_001132285.1 NZ_CP035245:c1583787-1583608 [Streptococcus pneumoniae]
MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGKLNKYTERGIRKQAGL

Nucleotide

• Length: 180 bp

>T117460 NZ_CP035245:c1583787-1583608 [Streptococcus pneumoniae]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCA
TATTAAAATGGAAAAGCAAGGGGAAAGACCTATCACAATCCTTCATGGTAAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16659.46 Da
• Isoelectric Point: 3.7220

>AT117460 WP_000961812.1 NZ_CP035245:c1583571-1583119 [Streptococcus pneumoniae]
MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAMGSEWLGLTVADLIESDGELPQPSDINSLSLIDNDPF
KDDEDFVSTYDLDKSFISMVSVDVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA

Nucleotide

• Length: 453 bp

>AT117460 NZ_CP035245:c1583571-1583119 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGCTAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACCATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Structures

Toxin

• AlphaFold DB: A0A0T9HEZ5

Antitoxin

• AlphaFold DB: A0A4J1XUY0