Detailed information of TA system TA117452

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 993979..995231
• Replicon: chromosome
• Accession: NZ_CP035243
• Organism: Streptococcus pneumoniae strain TVO_1901946

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: EQH37_RS05055
• Protein ID: WP_054365402.1
• Coordinates: 994455..995231 (+)
• Length: 259 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: B6ZCC4
• Locus tag: EQH37_RS05050
• Protein ID: WP_000579612.1
• Coordinates: 993979..994455 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQH37_RS05020 (EQH37_05320)	989320..989508	+	189	WP_000179769.1	hypothetical protein	-
EQH37_RS05025 (EQH37_05325)	989570..990328	+	759	WP_000262027.1	ATP-grasp domain-containing protein	-
EQH37_RS05030 (EQH37_05330)	990386..991240	+	855	WP_000900015.1	ADP-ribosylglycohydrolase family protein	-
EQH37_RS05035 (EQH37_05335)	991352..992236	+	885	WP_000710115.1	diacylglycerol kinase family lipid kinase	-
EQH37_RS05040 (EQH37_05340)	992399..993132	+	734	Protein_1006	alpha amylase N-terminal ig-like domain-containing protein	-
EQH37_RS05045 (EQH37_05345)	993109..993534	+	426	Protein_1007	hypothetical protein	-
EQH37_RS05050 (EQH37_05350)	993979..994455	+	477	WP_000579612.1	type II toxin-antitoxin system antitoxin PezA	Antitoxin
EQH37_RS05055 (EQH37_05355)	994455..995231	+	777	WP_054365402.1	type II toxin-antitoxin system toxin PezT	Toxin
EQH37_RS05060 (EQH37_05360)	995221..996978	+	1758	WP_000394602.1	AAA family ATPase	-
EQH37_RS05065 (EQH37_05365)	996971..998509	+	1539	WP_001279800.1	UvrD-helicase domain-containing protein	-
EQH37_RS05070 (EQH37_05375)	999403..999759	+	357	WP_001115086.1	SAG1252 family conjugative relaxosome accessory protein	-
EQH37_RS05075 (EQH37_05380)	999764..1000129	+	366	WP_000431637.1	MobC family plasmid mobilization relaxosome protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	969289..1095612	126323

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 259 a.a.
• Molecular weight: 29700.69 Da
• Isoelectric Point: 7.2008

>T117452 WP_054365402.1 NZ_CP035243:994455-995231 [Streptococcus pneumoniae]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSSVYDSKENTTSAADVLQELLFGEWSQVEKEML
RVVEERHKELEGKNSYGI

Nucleotide

• Length: 777 bp

>T117452 NZ_CP035243:994455-995231 [Streptococcus pneumoniae]
ATGGAAATCCAAGATTATACTGATAGTGAATTCAAACATGCTTTAGCAAGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAGCAACCTATAGCGATTTTGCTTGGAGGTCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGTGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGACAGTGTAGAATATACCAAAGATTTTGCAGGAAAAATGGTAGAGTCTTTAGTAACAAAATTGAGTAG
TTTGAGATACAATCTTTTGATAGAGGGAACTTTACGAACAGTTGATGTTCCAAAGAAAACAGCACAACTCTTGAAAAATA
AGGGATATGAAGTACAATTGGCCTTAATTGCGACAAAGCCTGAATTGTCGTATCTAAGTACTCTTATCCGTTATGAAGAA
CTGTACATTATCAATCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
TACACGACAATTGGAAGAACTGGCTATCTTTGAAAGAATTCAAATTTACCAACGAGATAGAAGTTCTGTATATGATTCAA
AAGAAAATACAACTTCAGCAGCAGATGTTCTTCAAGAGTTACTCTTTGGGGAGTGGAGTCAGGTAGAGAAAGAGATGTTG
AGGGTGGTGGAAGAAAGACATAAAGAGTTAGAAGGAAAAAATAGTTATGGAATTTAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18245.68 Da
• Isoelectric Point: 4.4188

>AT117452 WP_000579612.1 NZ_CP035243:993979-994455 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT117452 NZ_CP035243:993979-994455 [Streptococcus pneumoniae]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACACAACCCGAATTTGCACGAATTATAGGAATTTC
TCGAAATAGCTTGAGTCGTTATGAAAATGGAACTAGTTCAGTCTCTACGGAATTAATTGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTGAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTATTATCTCGACTCTATCGCTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTTTCTGATTTGATTCATACGAATATCTATTTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGTGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAGCGGATGGTAGCTTAA

Structures

Antitoxin

• AlphaFold DB: B6ZCC4