Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1595339..1596007 Replicon chromosome
Accession NZ_CP035238
Organism Streptococcus pneumoniae strain TVO_D39

Toxin (Protein)


Gene name hicA Uniprot ID B1I7Q0
Locus tag EQH42_RS07935 Protein ID WP_001132285.1
Coordinates 1595828..1596007 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID G0IC64
Locus tag EQH42_RS07930 Protein ID WP_000961810.1
Coordinates 1595339..1595791 (-) Length 151 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQH42_RS07905 (EQH42_08370) 1591229..1591972 - 744 WP_001188203.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
EQH42_RS07910 (EQH42_08375) 1591974..1592924 - 951 WP_000451131.1 50S ribosomal protein L11 methyltransferase -
EQH42_RS07915 (EQH42_08380) 1593062..1593490 - 429 WP_000418176.1 NUDIX hydrolase -
EQH42_RS07920 (EQH42_08385) 1593456..1594559 - 1104 WP_000719717.1 site-2 protease family protein -
EQH42_RS07925 (EQH42_08390) 1594578..1595048 - 471 WP_000257105.1 DUF3013 family protein -
EQH42_RS07930 (EQH42_08395) 1595339..1595791 - 453 WP_000961810.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EQH42_RS07935 (EQH42_08400) 1595828..1596007 - 180 WP_001132285.1 type II toxin-antitoxin system HicA family toxin Toxin
EQH42_RS07940 (EQH42_08405) 1596144..1596323 - 180 WP_001809903.1 hypothetical protein -
EQH42_RS07945 (EQH42_08415) 1596488..1596727 - 240 WP_000818079.1 hypothetical protein -
EQH42_RS07950 (EQH42_08425) 1596997..1598268 + 1272 WP_001113205.1 replication-associated recombination protein A -
EQH42_RS07960 (EQH42_08435) 1598815..1600134 - 1320 WP_000608887.1 glycoside hydrolase family 32 protein -
EQH42_RS07965 (EQH42_08440) 1600144..1600989 - 846 WP_000819520.1 carbohydrate ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(17-114)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6713.01 Da        Isoelectric Point: 11.2964

>T117440 WP_001132285.1 NZ_CP035238:c1596007-1595828 [Streptococcus pneumoniae]
MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGKLNKYTERGIRKQAGL

Download         Length: 180 bp

>T117440 NZ_CP035238:c1596007-1595828 [Streptococcus pneumoniae]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCA
TATTAAAATGGAAAAGCAAGGGGAAAGACCTATCACAATCCTTCATGGTAAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin


Download         Length: 151 a.a.        Molecular weight: 16659.46 Da        Isoelectric Point: 3.7220

>AT117440 WP_000961810.1 NZ_CP035238:c1595791-1595339 [Streptococcus pneumoniae]
MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAMGSEWLGITVADLIESDGELPQPSDINSLSLIDNDPF
KDDEDFVSTYDLDKSFISMVSVDVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA

Download         Length: 453 bp

>AT117440 NZ_CP035238:c1595791-1595339 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATTAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACTATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0T9HEZ5

Antitoxin

Source ID Structure
PDB 5YRZ
AlphaFold DB G0IC64

References