Detailed information of TA system TA117431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 977773..979010
• Replicon: chromosome
• Accession: NZ_CP035236
• Organism: Streptococcus pneumoniae strain TVO_1902282

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: A0A098ZIW8
• Locus tag: EQH44_RS04955
• Protein ID: WP_000405362.1
• Coordinates: 978249..979010 (+)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: EQH44_RS04950
• Protein ID: WP_000579613.1
• Coordinates: 977773..978249 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQH44_RS04930 (EQH44_05235)	972846..973700	+	855	WP_000900020.1	ADP-ribosylglycohydrolase family protein	-
EQH44_RS04935 (EQH44_05240)	973812..974696	+	885	WP_000710115.1	diacylglycerol kinase family lipid kinase	-
EQH44_RS04940 (EQH44_05245)	974859..976598	+	1740	Protein_987	glycoside hydrolase family 13 protein	-
EQH44_RS04945 (EQH44_05250)	976873..977328	+	456	WP_000500474.1	hypothetical protein	-
EQH44_RS04950 (EQH44_05255)	977773..978249	+	477	WP_000579613.1	type II toxin-antitoxin system antitoxin PezA	Antitoxin
EQH44_RS04955 (EQH44_05260)	978249..979010	+	762	WP_000405362.1	type II toxin-antitoxin system toxin PezT	Toxin
EQH44_RS04960	979038..979328	+	291	WP_001213954.1	hypothetical protein	-
EQH44_RS04965	979322..980194	+	873	WP_001811494.1	hypothetical protein	-
EQH44_RS04970 (EQH44_05275)	981013..981372	+	360	WP_000003745.1	SAG1252 family conjugative relaxosome accessory protein	-
EQH44_RS04975 (EQH44_05280)	981382..981747	+	366	WP_000263048.1	MobC family plasmid mobilization relaxosome protein	-
EQH44_RS04980 (EQH44_05285)	981734..983584	+	1851	WP_000261804.1	SAG1250 family conjugative relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	953898..1091051	137153

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 29129.16 Da
• Isoelectric Point: 7.1308

>T117431 WP_000405362.1 NZ_CP035236:978249-979010 [Streptococcus pneumoniae]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
QVGEKRLNELLEK

Nucleotide

• Length: 762 bp

>T117431 NZ_CP035236:978249-979010 [Streptococcus pneumoniae]
ATGGAAATCCAAGATTATACTGATAGTGAATTCAAACATGCTTTAGCAAGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAACAACCTATAGCGATTTTGCTTGGAGGTCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGTGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGACAGTGTAGAATATACCAAAGATTTTGCAGGAAAAATGGTAGAGTCTTTAGTAACAAAATTGAGTAG
TTTGAGATACAATCTTTTGATAGAGGGAACTTTACGAACAGTTGATGTTCCAAAGAAAACAGCACAACTCTTGAAAAATA
AGGGATATGAAGTACAATTGGCCTTAATTGCGACAAAGCCTGAATTGTCGTATCTAAGTACTCTTATCCGTTATGAAGAA
CTGTACATTATCAATCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
TACACGACAATTGGAAGAACTGGCTATCTTTGAAAGAATTCAAATTTACCAACGAGATAGAAGTTGTGTATATGATTCAA
AAGAAAATACAACTTCAGCAGCAGATGTTCTTCAAGAGTTACTGTTTGGGGAGTGGAGTCAGGTAGAGAAGGAGATGTTG
CAGGTGGGGGAAAAGAGACTTAATGAATTACTTGAAAAATAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18249.67 Da
• Isoelectric Point: 4.3462

>AT117431 WP_000579613.1 NZ_CP035236:977773-978249 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT117431 NZ_CP035236:977773-978249 [Streptococcus pneumoniae]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACACAACCTGAATTTGCACGAATTATAGGAATTTC
TCGAAATAGCTTGAGTCGTTATGAAAATGGAACTAGTTCAGTCTCTACGGAATTAATTGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTGAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTATTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGACTTGATTCAGACAAATATCTATTTAGTAGAAAATTTTGATG
AAATAGAGAGATATAGCGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAGCGGATGGTAGCTTAA

Structures

Toxin

• AlphaFold DB: A0A098ZIW8