Detailed information of TA system TA117423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1476928..1477847
• Replicon: chromosome
• Accession: NZ_CP035232
• Organism: Bacillus glycinifermentans strain SRCM103574

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A0A1C3SBI7
• Locus tag: EQZ20_RS07635
• Protein ID: WP_046131131.1
• Coordinates: 1477101..1477847 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: -
• Locus tag: EQZ20_RS07630
• Protein ID: WP_046131317.1
• Coordinates: 1476928..1477101 (-)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQZ20_RS07610	1471991..1473088	+	1098	WP_046131134.1	mannonate dehydratase	-
EQZ20_RS07615	1473064..1473912	+	849	WP_046131133.1	SDR family oxidoreductase	-
EQZ20_RS07620	1473925..1474944	+	1020	WP_046131132.1	zinc-binding alcohol dehydrogenase family protein	-
EQZ20_RS07625	1474979..1476252	+	1274	Protein_1462	MFS transporter	-
EQZ20_RS07630	1476928..1477101	-	174	WP_046131317.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
EQZ20_RS07635	1477101..1477847	-	747	WP_046131131.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
EQZ20_RS07640	1477963..1478961	-	999	WP_046131130.1	inorganic phosphate transporter	-
EQZ20_RS07645	1478974..1479591	-	618	WP_006638544.1	DUF47 domain-containing protein	-
EQZ20_RS07650	1479915..1481675	+	1761	WP_046131129.1	gamma-glutamyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-57)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29645.05 Da
• Isoelectric Point: 4.6123

>T117423 WP_046131131.1 NZ_CP035232:c1477847-1477101 [Bacillus glycinifermentans]
MLLFYQFSVWVIVLALALYVFAVWRFEKKMKEKTIAIRKTWYLLYVIGAALYWTYDPQSIFSNWLHYLIVAVFFTLTDAF
IFLNAYFKKLGTNELATDTRELLEENNDLLHTYQNRLKTYQYLLKNEPIHIYYGDIEAYAEGIEKLIKRFAEKMNISAAL
CEYNSQESKDHLLEHMENRFAVQEKLDRKDVYYEENGKMVLIPFSIHEYDYVMKLTSEDLVTEFDYLLFTSLTSIYDLLL
PNEEEGDE

Nucleotide

• Length: 747 bp

>T117423 NZ_CP035232:c1477847-1477101 [Bacillus glycinifermentans]
ATGCTGCTTTTTTATCAATTTTCAGTCTGGGTGATCGTCCTCGCCTTAGCCTTGTACGTTTTTGCCGTCTGGCGTTTTGA
AAAAAAGATGAAAGAAAAAACGATCGCAATCCGGAAAACATGGTATCTCCTTTATGTGATCGGCGCCGCGCTTTACTGGA
CGTACGATCCGCAGTCCATTTTCAGCAATTGGCTCCATTACTTGATCGTCGCCGTCTTTTTTACGTTGACTGATGCGTTT
ATTTTCTTAAACGCCTACTTCAAAAAACTCGGCACGAACGAATTGGCCACAGACACAAGAGAGCTTCTTGAAGAAAACAA
CGACCTGCTCCACACGTATCAAAACAGGCTCAAAACGTACCAATACCTATTGAAAAACGAACCGATCCACATCTACTATG
GAGATATAGAAGCTTACGCTGAAGGCATTGAAAAACTCATCAAACGGTTTGCCGAAAAAATGAATATATCCGCCGCACTA
TGCGAATATAATTCACAAGAGAGCAAAGATCATTTGCTCGAACATATGGAAAACCGTTTCGCTGTTCAGGAAAAGCTCGA
CCGGAAAGACGTGTATTACGAAGAAAACGGAAAAATGGTCCTGATTCCATTCTCGATTCATGAATACGACTATGTGATGA
AACTGACATCCGAGGACCTTGTCACTGAGTTTGATTATCTGCTGTTTACTTCTTTAACCAGCATCTATGATCTTCTGCTA
CCAAACGAAGAGGAAGGTGACGAATGA

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6810.82 Da
• Isoelectric Point: 10.7543

>AT117423 WP_046131317.1 NZ_CP035232:c1477101-1476928 [Bacillus glycinifermentans]
MEPVFQQDKDRSTGRGTCKVFKKQSKTHIAKYEVSPHTERIFRQNARLIDEYKRKKA

Nucleotide

• Length: 174 bp

>AT117423 NZ_CP035232:c1477101-1476928 [Bacillus glycinifermentans]
ATGGAGCCGGTTTTTCAGCAAGACAAAGACCGTTCAACGGGGCGCGGCACATGTAAAGTATTCAAAAAGCAATCAAAAAC
CCACATCGCCAAATACGAAGTCAGCCCCCACACCGAACGGATTTTCAGGCAAAATGCCCGGCTGATCGATGAGTATAAAC
GAAAAAAAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A1C3SBI7