Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 603736..604372 Replicon chromosome
Accession NZ_CP035232
Organism Bacillus glycinifermentans strain SRCM103574

Toxin (Protein)


Gene name mazF Uniprot ID M5P3Q9
Locus tag EQZ20_RS02985 Protein ID WP_003179128.1
Coordinates 604022..604372 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A6I7F8B4
Locus tag EQZ20_RS02980 Protein ID WP_026585993.1
Coordinates 603736..604017 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQZ20_RS02960 598901..600381 + 1481 Protein_551 PH domain-containing protein -
EQZ20_RS02965 600380..600976 - 597 Protein_552 rhomboid family intramembrane serine protease -
EQZ20_RS02970 601258..602274 + 1017 WP_099047092.1 outer membrane lipoprotein carrier protein LolA -
EQZ20_RS02975 602468..603627 + 1160 Protein_554 alanine racemase -
EQZ20_RS02980 603736..604017 + 282 WP_026585993.1 hypothetical protein Antitoxin
EQZ20_RS02985 604022..604372 + 351 WP_003179128.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EQZ20_RS02990 604446..605315 + 870 WP_173427511.1 STAS domain-containing protein -
EQZ20_RS02995 605318..605683 + 366 WP_046132197.1 STAS domain-containing protein -
EQZ20_RS03000 605686..606087 + 402 WP_046132196.1 anti-sigma regulatory factor -
EQZ20_RS03005 606098..607105 + 1008 WP_046132195.1 PP2C family protein-serine/threonine phosphatase -
EQZ20_RS03010 607164..607490 + 327 WP_046132194.1 anti-sigma factor antagonist -
EQZ20_RS03015 607490..607972 + 483 WP_046132193.1 anti-sigma B factor RsbW -
EQZ20_RS03020 607938..608729 + 792 WP_046132218.1 RNA polymerase sigma factor SigB -
EQZ20_RS03025 608726..609325 + 600 WP_046132192.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12990.03 Da        Isoelectric Point: 5.7234

>T117422 WP_003179128.1 NZ_CP035232:604022-604372 [Bacillus glycinifermentans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Download         Length: 351 bp

>T117422 NZ_CP035232:604022-604372 [Bacillus glycinifermentans]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGATCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTATGGTTTTGAAAGAGATTCCGTTATATTGCTCGAACAAATC
CGGACGATTGATAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTTAACGAAGCCTT
ACAAATCAGTTTGGCACTAATCGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10667.07 Da        Isoelectric Point: 4.6920

>AT117422 WP_026585993.1 NZ_CP035232:603736-604017 [Bacillus glycinifermentans]
MSESSATTEIMIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Download         Length: 282 bp

>AT117422 NZ_CP035232:603736-604017 [Bacillus glycinifermentans]
TTGTCTGAATCCAGCGCTACAACTGAAATTATGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCCGAAATGAACTGATTTATCAAGCCACAAAAATGTATTTACGTGAACGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCAAAAATCAATTTAAACATCTCCTCAGAGGCACAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I7FHI4

Antitoxin

Source ID Structure
AlphaFold DB A0A6I7F8B4

References