Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 548542..549178 Replicon chromosome
Accession NZ_CP035231
Organism Bacillus subtilis strain SRCM103571

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag EQZ01_RS02860 Protein ID WP_003156187.1
Coordinates 548828..549178 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag EQZ01_RS02855 Protein ID WP_003225183.1
Coordinates 548542..548823 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQZ01_RS02835 544901..545500 - 600 WP_015252769.1 rhomboid family intramembrane serine protease -
EQZ01_RS02840 545595..545960 + 366 WP_015252768.1 holo-ACP synthase -
EQZ01_RS02845 546126..547142 + 1017 WP_080019556.1 outer membrane lipoprotein carrier protein LolA -
EQZ01_RS02850 547257..548426 + 1170 WP_015252766.1 alanine racemase -
EQZ01_RS02855 548542..548823 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
EQZ01_RS02860 548828..549178 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EQZ01_RS02865 549293..550117 + 825 WP_015252765.1 RsbT co-antagonist protein RsbRA -
EQZ01_RS02870 550122..550487 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
EQZ01_RS02875 550491..550892 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
EQZ01_RS02880 550904..551911 + 1008 WP_015252763.1 phosphoserine phosphatase RsbU -
EQZ01_RS02885 551973..552302 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
EQZ01_RS02890 552299..552781 + 483 WP_003234299.1 anti-sigma B factor RsbW -
EQZ01_RS02895 552747..553535 + 789 WP_048654765.1 RNA polymerase sigma factor SigB -
EQZ01_RS02900 553535..554134 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T117420 WP_003156187.1 NZ_CP035231:548828-549178 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T117420 NZ_CP035231:548828-549178 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT117420 WP_003225183.1 NZ_CP035231:548542-548823 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT117420 NZ_CP035231:548542-548823 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References