Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 510808..511444 Replicon chromosome
Accession NZ_CP035230
Organism Bacillus subtilis strain SRCM103551

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag EQY76_RS02640 Protein ID WP_003156187.1
Coordinates 511094..511444 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag EQY76_RS02635 Protein ID WP_003225183.1
Coordinates 510808..511089 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQY76_RS02615 507167..507766 - 600 WP_032722928.1 rhomboid family intramembrane serine protease -
EQY76_RS02620 507861..508226 + 366 WP_015252768.1 holo-ACP synthase -
EQY76_RS02625 508392..509408 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
EQY76_RS02630 509523..510692 + 1170 WP_015252766.1 alanine racemase -
EQY76_RS02635 510808..511089 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
EQY76_RS02640 511094..511444 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EQY76_RS02645 511559..512383 + 825 WP_080320324.1 RsbT co-antagonist protein RsbRA -
EQY76_RS02650 512388..512753 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
EQY76_RS02655 512757..513158 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
EQY76_RS02660 513170..514177 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
EQY76_RS02665 514239..514568 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
EQY76_RS02670 514565..515047 + 483 WP_003234299.1 anti-sigma B factor RsbW -
EQY76_RS02675 515013..515801 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
EQY76_RS02680 515801..516400 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T117418 WP_003156187.1 NZ_CP035230:511094-511444 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T117418 NZ_CP035230:511094-511444 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT117418 WP_003225183.1 NZ_CP035230:510808-511089 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT117418 NZ_CP035230:510808-511089 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References