Detailed information of TA system TA117417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1403294..1404213
• Replicon: chromosome
• Accession: NZ_CP035228
• Organism: Bacillus licheniformis strain SRCM103529

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: T5HIF3
• Locus tag: EQY74_RS07270
• Protein ID: WP_003180879.1
• Coordinates: 1403467..1404213 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: Q65L00
• Locus tag: EQY74_RS07265
• Protein ID: WP_011197812.1
• Coordinates: 1403294..1403467 (-)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQY74_RS07245	1398719..1399816	+	1098	WP_017474398.1	mannonate dehydratase	-
EQY74_RS07250	1399792..1400640	+	849	WP_009328728.1	SDR family oxidoreductase	-
EQY74_RS07255	1400667..1401680	+	1014	WP_017474397.1	zinc-binding alcohol dehydrogenase family protein	-
EQY74_RS07260	1401733..1403004	+	1272	WP_011197811.1	MFS transporter	-
EQY74_RS07265	1403294..1403467	-	174	WP_011197812.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
EQY74_RS07270	1403467..1404213	-	747	WP_003180879.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
EQY74_RS07275	1404324..1405322	-	999	WP_009328723.1	inorganic phosphate transporter	-
EQY74_RS07280	1405335..1405952	-	618	WP_003180884.1	DUF47 domain-containing protein	-
EQY74_RS07285	1406285..1408042	+	1758	WP_017474396.1	gamma-glutamyltransferase	-
EQY74_RS07290	1408179..1408823	+	645	WP_009328721.1	YesL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-57)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29391.80 Da
• Isoelectric Point: 4.5367

>T117417 WP_003180879.1 NZ_CP035228:c1404213-1403467 [Bacillus licheniformis]
MLLFYQFLVWLIVLALALYVAAVWRFEKQLAEKTVAIRKTWYLLYVIGAVIYWTHDPQSIFTNPLHYLIVAVFFTLTDAF
IFLNAYFKKLGSSELATDTRMLLEENNDLLHTYQNRLKTFQYLLKNEPIHIYYGNIEAYAEGIEKLIKRFAEKMNISAAL
CEYNSEESKDHLLEHMENRFDVQEKLDRKDVYYEENGKMVLIPFSIHDFDYVMKLTSEDLVTEFDYLLFTSLTSIYDLLL
PNEEEGDD

Nucleotide

• Length: 747 bp

>T117417 NZ_CP035228:c1404213-1403467 [Bacillus licheniformis]
ATGCTGCTATTTTATCAATTCCTTGTTTGGCTTATTGTTTTGGCGCTGGCTCTGTACGTAGCTGCCGTGTGGCGTTTTGA
AAAACAGCTCGCCGAAAAAACGGTCGCCATCAGAAAAACGTGGTACCTTCTTTATGTCATCGGCGCCGTGATATATTGGA
CGCACGATCCGCAGTCAATTTTCACAAACCCGCTTCATTACCTGATCGTTGCCGTTTTTTTCACGTTGACAGACGCTTTT
ATTTTCTTAAATGCCTACTTTAAAAAGCTCGGCAGCTCTGAACTCGCGACAGATACAAGAATGCTTCTCGAAGAAAACAA
CGACCTCCTCCACACGTATCAAAACAGGCTGAAAACGTTTCAATACCTATTGAAAAACGAACCGATCCACATCTATTATG
GAAATATAGAAGCGTATGCAGAAGGCATCGAAAAGCTCATCAAACGGTTTGCCGAAAAAATGAATATATCCGCTGCACTT
TGCGAATATAATTCAGAAGAAAGCAAGGATCATTTGCTTGAGCATATGGAAAACCGGTTCGATGTTCAAGAAAAGCTTGA
CCGCAAAGACGTTTATTATGAAGAAAACGGAAAAATGGTCCTCATTCCTTTTTCCATCCACGATTTTGACTATGTCATGA
AGTTAACCTCAGAAGACCTTGTCACAGAATTTGATTATCTCCTGTTTACTTCTTTAACAAGCATCTATGATCTTCTGCTA
CCAAACGAAGAGGAAGGTGACGACTGA

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6971.08 Da
• Isoelectric Point: 10.7815

>AT117417 WP_011197812.1 NZ_CP035228:c1403467-1403294 [Bacillus licheniformis]
MEPAFQQDQESVKKRLAFKMFKRQSKTNIAKYEVSPYTERIFRQNERLIGEYKRKKA

Nucleotide

• Length: 174 bp

>AT117417 NZ_CP035228:c1403467-1403294 [Bacillus licheniformis]
ATGGAGCCTGCTTTTCAGCAAGACCAGGAAAGCGTAAAAAAGCGCCTCGCCTTTAAAATGTTTAAAAGGCAATCCAAAAC
CAATATCGCGAAATACGAAGTCAGCCCCTATACCGAACGGATTTTCCGGCAAAACGAACGGCTGATCGGCGAGTATAAAA
GAAAAAAAGCTTAA

Structures

Toxin

• AlphaFold DB: T5HIF3

Antitoxin

• AlphaFold DB: Q65L00