Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 561106..561742 Replicon chromosome
Accession NZ_CP035228
Organism Bacillus licheniformis strain SRCM103529

Toxin (Protein)


Gene name mazF Uniprot ID M5P3Q9
Locus tag EQY74_RS02805 Protein ID WP_003179128.1
Coordinates 561392..561742 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M5PDU2
Locus tag EQY74_RS02800 Protein ID WP_006638778.1
Coordinates 561106..561387 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQY74_RS02780 556221..557702 + 1482 WP_009330132.1 PH domain-containing protein -
EQY74_RS02785 557699..558298 - 600 WP_003179118.1 rhomboid family intramembrane serine protease -
EQY74_RS02790 558641..559600 + 960 WP_152847888.1 outer membrane lipoprotein carrier protein LolA -
EQY74_RS02795 559825..560994 + 1170 WP_026080763.1 alanine racemase -
EQY74_RS02800 561106..561387 + 282 WP_006638778.1 hypothetical protein Antitoxin
EQY74_RS02805 561392..561742 + 351 WP_003179128.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EQY74_RS02810 561860..562687 + 828 WP_003179130.1 RsbT co-antagonist protein RsbRA -
EQY74_RS02815 562691..563056 + 366 WP_003179132.1 STAS domain-containing protein -
EQY74_RS02820 563059..563460 + 402 WP_003179135.1 anti-sigma regulatory factor -
EQY74_RS02825 563471..564478 + 1008 WP_003179137.1 PP2C family protein-serine/threonine phosphatase -
EQY74_RS02830 564537..564863 + 327 WP_003179140.1 anti-sigma factor antagonist -
EQY74_RS02835 564863..565348 + 486 WP_003179142.1 anti-sigma B factor RsbW -
EQY74_RS02840 565314..566105 + 792 WP_003179144.1 RNA polymerase sigma factor SigB -
EQY74_RS02845 566102..566701 + 600 WP_003179145.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12990.03 Da        Isoelectric Point: 5.7234

>T117416 WP_003179128.1 NZ_CP035228:561392-561742 [Bacillus licheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Download         Length: 351 bp

>T117416 NZ_CP035228:561392-561742 [Bacillus licheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGACTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10649.03 Da        Isoelectric Point: 4.6920

>AT117416 WP_006638778.1 NZ_CP035228:561106-561387 [Bacillus licheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Download         Length: 282 bp

>AT117416 NZ_CP035228:561106-561387 [Bacillus licheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTACGCGAACGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATCAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I7FHI4

Antitoxin

Source ID Structure
AlphaFold DB M5PDU2

References