Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 508657..509293 Replicon chromosome
Accession NZ_CP035226
Organism Bacillus subtilis strain SRCM103517

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag EQW70_RS02630 Protein ID WP_003156187.1
Coordinates 508943..509293 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag EQW70_RS02625 Protein ID WP_003225183.1
Coordinates 508657..508938 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQW70_RS02605 505016..505615 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
EQW70_RS02610 505710..506075 + 366 WP_014475874.1 holo-ACP synthase -
EQW70_RS02615 506241..507257 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
EQW70_RS02620 507372..508541 + 1170 WP_015252766.1 alanine racemase -
EQW70_RS02625 508657..508938 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
EQW70_RS02630 508943..509293 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EQW70_RS02635 509408..510232 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
EQW70_RS02640 510237..510602 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
EQW70_RS02645 510606..511007 + 402 WP_003225192.1 serine/threonine-protein kinase RsbT -
EQW70_RS02650 511019..512026 + 1008 WP_014478900.1 phosphoserine phosphatase RsbU -
EQW70_RS02655 512095..512424 + 330 WP_014475877.1 anti-sigma factor antagonist RsbV -
EQW70_RS02660 512421..512903 + 483 WP_095010756.1 anti-sigma B factor RsbW -
EQW70_RS02665 512869..513657 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
EQW70_RS02670 513657..514256 + 600 WP_021481701.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T117404 WP_003156187.1 NZ_CP035226:508943-509293 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T117404 NZ_CP035226:508943-509293 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT117404 WP_003225183.1 NZ_CP035226:508657-508938 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT117404 NZ_CP035226:508657-508938 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References