Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 3271..3914 Replicon plasmid pTH164-1
Accession NZ_CP035212
Organism Klebsiella pneumoniae strain TH164

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag EQH51_RS27245 Protein ID WP_000754566.1
Coordinates 3271..3687 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag EQH51_RS27250 Protein ID WP_001261276.1
Coordinates 3684..3914 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQH51_RS27220 129..212 - 84 Protein_0 SOS response-associated peptidase -
EQH51_RS27230 853..1929 - 1077 WP_000227969.1 IS110 family transposase -
EQH51_RS27240 2211..3067 - 857 Protein_2 IS3-like element ISEc15 family transposase -
EQH51_RS27245 3271..3687 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
EQH51_RS27250 3684..3914 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
EQH51_RS27260 4488..4838 + 351 WP_000493378.1 hypothetical protein -
EQH51_RS27265 4889..5632 + 744 WP_000129823.1 hypothetical protein -
EQH51_RS27270 5629..6405 + 777 WP_000015958.1 site-specific integrase -
EQH51_RS27275 6463..6720 - 258 WP_000764642.1 hypothetical protein -
EQH51_RS28560 6849..6953 - 105 WP_032409716.1 hypothetical protein -
EQH51_RS27280 7483..8349 + 867 WP_004118283.1 replication initiation protein -
EQH51_RS27285 8526..8795 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / sul2 / floR / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aac(3)-IId / qnrS1 / blaTEM-1B - 1..69596 69596


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T117401 WP_000754566.1 NZ_CP035212:c3687-3271 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T117401 NZ_CP035212:c3687-3271 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT117401 WP_001261276.1 NZ_CP035212:c3914-3684 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT117401 NZ_CP035212:c3914-3684 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References