Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 12522..13165 Replicon plasmid pTH114-1
Accession NZ_CP035209
Organism Klebsiella quasipneumoniae strain TH114

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag EQH50_RS25420 Protein ID WP_001044770.1
Coordinates 12749..13165 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag EQH50_RS25415 Protein ID WP_001261282.1
Coordinates 12522..12752 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQH50_RS25380 7724..7993 + 270 WP_000339857.1 hypothetical protein -
EQH50_RS25385 8170..9036 - 867 WP_004118283.1 replication initiation protein -
EQH50_RS26085 9566..9670 + 105 WP_032409716.1 hypothetical protein -
EQH50_RS25390 9799..10056 + 258 WP_000764642.1 hypothetical protein -
EQH50_RS25395 10114..10890 - 777 WP_020956881.1 site-specific integrase -
EQH50_RS25400 10902..11588 - 687 WP_020956882.1 hypothetical protein -
EQH50_RS25405 11652..11945 - 294 WP_020956883.1 hypothetical protein -
EQH50_RS25410 12104..12565 - 462 WP_074193041.1 hypothetical protein -
EQH50_RS25415 12522..12752 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
EQH50_RS25420 12749..13165 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
EQH50_RS25425 13239..14801 + 1563 WP_004206609.1 AAA family ATPase -
EQH50_RS25430 14786..15808 + 1023 WP_000361404.1 helicase UvrD -
EQH50_RS25435 16346..17260 + 915 WP_031592169.1 HNH endonuclease -
EQH50_RS25440 17446..17796 - 351 WP_004187110.1 DUF305 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid dfrA12 / aadA2 / floR / sul2 / blaTEM-1B / tet(A) - 1..73414 73414


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T117383 WP_001044770.1 NZ_CP035209:12749-13165 [Klebsiella quasipneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T117383 NZ_CP035209:12749-13165 [Klebsiella quasipneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT117383 WP_001261282.1 NZ_CP035209:12522-12752 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT117383 NZ_CP035209:12522-12752 [Klebsiella quasipneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References