Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2663910..2664529 Replicon chromosome
Accession NZ_CP035207
Organism Klebsiella quasipneumoniae strain TH114

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag EQH50_RS13025 Protein ID WP_002892050.1
Coordinates 2664311..2664529 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag EQH50_RS13020 Protein ID WP_002892066.1
Coordinates 2663910..2664284 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQH50_RS13010 2659063..2660256 + 1194 WP_004204747.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
EQH50_RS13015 2660279..2663425 + 3147 WP_152651475.1 multidrug efflux RND transporter permease subunit AcrB -
EQH50_RS13020 2663910..2664284 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
EQH50_RS13025 2664311..2664529 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
EQH50_RS13030 2664688..2665254 + 567 WP_152651476.1 maltose O-acetyltransferase -
EQH50_RS25960 2665226..2665348 - 123 WP_194164719.1 hypothetical protein -
EQH50_RS13035 2665391..2665861 + 471 WP_049093338.1 YlaC family protein -
EQH50_RS13040 2665830..2667287 - 1458 WP_049117056.1 PLP-dependent aminotransferase family protein -
EQH50_RS13045 2667388..2668086 + 699 WP_152651477.1 GNAT family N-acetyltransferase -
EQH50_RS13050 2668083..2668223 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
EQH50_RS13055 2668223..2668486 - 264 WP_004204754.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T117379 WP_002892050.1 NZ_CP035207:2664311-2664529 [Klebsiella quasipneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T117379 NZ_CP035207:2664311-2664529 [Klebsiella quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT117379 WP_002892066.1 NZ_CP035207:2663910-2664284 [Klebsiella quasipneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT117379 NZ_CP035207:2663910-2664284 [Klebsiella quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References